不育患者精浆α-1,4糖苷酶活性与精液参数的关系

Relationship between the seminal main parameters and seminal plasma alpha-1,4 glycosidase activity in infertile patients

目的 探讨精浆 α-1 ,4糖苷酶活性与精液参数之间的关系。 方法 分光光度比色法测定精浆 α-1 ,4糖苷酶活性及进行精液常规分析。 结果 2 90 2例男性不育者精浆α-1 ,4糖苷酶活性异常率为 3 8.87%。该酶活性与精子密度、精子活率、a,b级精子活力和顶体酶活性呈显著正相关 ( r分别为 0 .460、0 .1 2 2、0 .0 86和 0 .2 3 0 ,P均 <0 .0 0 1 ) ,而与精液量、精液 p H、液化时间和畸形精子率无显著相关 ( P>0 .0 5)。 α-1 ,4糖苷酶活性正常组精子密度、活率、a,b级精子活力和精子顶体酶活性均明显高于 α-1 ,4糖苷酶活性异常组 ( P<0 .0 0 1 )。以常规精液分析法 ( RSA)主要参数正常与否分成的两组间α-1 ,4糖苷酶活性差异有显著性 ( P<0 .0 0 1 )。 结论 α-1 ,4糖苷酶活性对精子密度、活率、a,b级精子活力和顶体酶活性均有明显影响 ,对精液量、精液 p H。

Objective: In order to study the relationship between alpha 1,4 glycosidase activity of human seminal plasma and the seminal main parameters in infertile man. Methods: The alpha 1,4 glycosidase activity was determined by spectrophotometry and the seminal main parameters were measured and analyzed by using routine semen analysis in 2 902 infertile men. Results: The abnormal rate of alpha 1,4 glycosidase activity in 2 902 infertile semen samples was 38.87%. Significant positive correlations were found between the alpha 1,4 glycosidase activity and sperm concentration, sperm viability, sperm motility(a,b grade), and sperm acrosin activity ( P <0.001). There was no significant correlation between the alpha 1,4 glycosidase activity and semen volume, semen pH, liquefied duration, and the rate of abnormal morphological sperms ( P >0.05). Sperm concentration, sperm viability, sperm motility (a,b grade) and sperm acrosin activity in normal alpha 1,4 glycosidase activity group were higher than those in abnormal alpha 1,4 glycosidase activity group. The comparison of alpha 1,4 glycosidase activity between two groups with normal or abnormal seminal parameters showed that the alpha 1,4 glycosidase activity significantly decreased when seminal parameters were abnormal ( P < 0.001). Conclusion: The alpha 1,4 glycosidase activity of human seminal plasma could affect sperm concentration, sperm viability, sperm motility(a,b grade), and sperm acrosin activity.