苯那普利减轻大鼠肾小球硬化及对血小板源性生长因子表达的影响

Lightening of glomerulosclerosis by benalapril and its effect on the expression of platelet-derived growth factor

目的 进一步探讨血管紧张素转化酶抑制剂 (ACEI)减轻肾小球硬化的机理。方法 3 6只雄性大鼠分为对照组、模型组和治疗组。模型组和治疗组行单侧肾切除大鼠加阿霉素注射 ,治疗组再给予每日苯那普利 6mg .kg- 1 灌胃 1次 ,共 12周。免疫组化法检测肾组织血小板源性生长因子 BB(PDGF BB)的表达 ,并进行半定量分析。计算不同组别肾小球硬化指数。结果 模型组较对照组肾小球硬化明显 ,PDGF BB表达明显增强 (P <0 .0 11) ;苯那普利治疗组肾小球硬化较模型组明显减轻 ,PDGF BB表达亦减弱 (P <0 .0 1)。结论 大鼠阿霉素肾病肾小球硬化时 ,PDGF BB表达增强 ,PDGF BB参与肾小球硬化的进展 ;ACEI可能通过降低PDGF

Objective To inquire into the mechanism of angiotensin converting enzyme inhibitor(ACEI) benazepril in lightening glomerulosclerosis. Methods Thirty six male rats were divided into control group, model group and treatment group with benazepril.Uninephrectomy and the injection of adriamycin induced the rats model with glomerulosclerosis in model group and treatment group. Benazepril(6mg·kg -1 ) was delivered daily by gavage in rats of treatment group for 12 weeks.Immunohistochemistry was utilized to detect the expression of platelet derivedgrowth factor BB (PDGF BB) and the level of PDGF BB was hemi quantitated. Glomerulosclerosis index was counted. Results Compared with those of the control group, the kidneys of the model group had more severe glomerulosclerosis and higher level of expression of PDGF BB( P <0.01). The degree of glomerulosclerosis and the level of expression of PDGF BB were ameliorated by benazepril treatment( P <0.01).Conclusion The level of PDGF BB is high in the kidney of rats with adriamycin induced nephritic glomerulosclerosis. PDGF BB takes part in glomerulosclerosis. ACEI benazepril may suppress the expression of PDGF BB so as to postpone the process of glomerulosclerosis.