ras基因和PCNA与胆囊癌预后的关系

Mutation of ras gene and overexpresion of PCNA as prognostic parameters in gallbladder cancer

目的 以ras基因和PCNA作为胆囊癌复发和预后的相关指标 ,探讨其与胆囊癌预后的关系。方法 PCNA用免疫组织化学ABC染色法测定 ;ras基因突变用PCR RFLP (限制片段长度多态性 )方法分析。结果 胆囊癌的预后和组织学分型、Nevin分期和手术治疗的效果有关 ;NevinIV ,V期的胆囊癌预后差 ,根治性手术能显著提高生存率。合并ras基因突变的病例比没有合并ras基因突变者其死亡危险度高 1.62倍。PCNA强阳性者死亡危险度比低表达者高 2 .2倍 (P <0 .0 5 )。结论 胆囊癌的组织学分型、Nevin分期、ras基因及PCNA可以作为胆囊癌预后的相关指标 ,有助于临床为胆囊癌患者制定手术方案和评估手术后的生存情况。

Objective To estimative the relationship between ras gene mutation,PCNA overexpression and the recurrence and prognosis of gallbladder cancer.Methods PCNA was studied by ABC immunohistology, ras gene mutation by PCR-RELP analysis.Results Histology,Nevin staging and operative methods were relevant to the prognosis of gallbladder cancer.The patient with stag IV,V gallbladder cancer had a poor prognosis;radical operations could prolong patients survival time.The mortal risk of patients with ras gene mutation was 1.62 times higher than that without ras gene mutation,with high overexpression of PCNA was 2.2 times higher than that with low expression of PCNA. Conclusions Differentiation degree, Nevin′s staging, mutation of ras gene and overexpresion of PCNA could be the prognostic factors in gallbladder cancer,and could help to select the operative procedure and to eluvate the surrival.