胆红素/HDEHP反相微乳液的制备及应用

Preparation and Application of the Reversed Microemulsion Containing Bilirubin/HDEHP

制备了部分皂化的含胆红素 (H2 BR) /二 (2 乙基已基 )磷酸酯 (HDEHP) /氯仿 /苯 /氨水的反相微乳液 ,并通过透射电镜、电导率仪和动态激光光散射仪等对所制备的微乳液进行了表征。结果表明 ,苯和氯仿的体积比为 7∶3的混合溶液为该微乳液形成的最佳有机溶剂 ,1 0 0mol/L的氨水为最有效皂化剂 ;形成微乳液的H2 BR和HDEHP的摩尔比随 2个表面活性剂总浓度的增加而减小 ;H2 BR量的多少对微乳液内增溶的水量影响较大。微乳液内的水滴直径在 6 0～ 12 0nm范围内 ,以此微乳液作为细胞膜模拟体系 ,制备了含CaCl2 /H2 BR/HDEHP/氯仿 /苯 /氨水的新微乳液 ,并进一步诱导和调控了胆红素钙的形成 ,发生了类似生物体内的矿化过程。

Bilirubin(H 2BR) and di-(2-ethylhexyl) phosphoric acid(HDEHP) were dissolved in chloroform/benzene mixture and partially saponified by ammonia to prepare reversed microemulsions which were characterized by TEM, conductometry and dynamic laser-scattering. The results showed that the mixture of benzene and chloroform in volume ratio of 7∶3 was an optimal solvent and ammonia with concentration of 1.00 mol/L was the best saponifier for the formation of microemulsions. The molar ratio of H 2BR to HDEHP decreased with the increase in the total concentration of surfactants. The content of H 2BR affected greatly the amount of water in microemulsions. The diameter of water pool in microemulsions ranged from 60 nm to 120 nm, which were used to simulate a cell membrane and to produce a new reversed microemulsions containing CaCl 2/H 2BR/HDEHP. Calcium bilirubinate could be induced and controlled by both surfactants in the system, similar to that of biomineralization process in organism.