HSA-硅钨杂多酸缔合纳米微粒体系的荧光猝灭

Fluorescence Quenching of HSA-Silicotungstic Heteropolyacid Associated Nanoparticle System

在 pH =7 4 0Tris缓冲溶液中 ,硅钨杂多酸 (SiW )在 2 6 0nm有 1吸收峰 ;人血清白蛋白 (HSA)在35 0nm处有 1荧光峰。当HSA与SiW存在时 ,二者形成粒径约 5 0nm的缔合纳米微粒 ,导致 4 70nm处瑞利散射 (RS)光信号增强及 35 0nm处荧光猝灭。RS光谱和透射电镜研究结果表明 ,HSA SiW缔合纳米微粒和界面的形成是导致体系荧光猝灭和RS增强的根本原因。

In a Tris buffer solution(pH=7.40), there are an absorption peak at 260 nm for silicotungstic heteropolyacid(SiW) system and independently a fluorescence peak at 350 nm for human serum albumin(HSA) system. When both HSA and SiW solutions are mixed, ion association nanoparticles in size of 50 nm are formed. As a result Rayleigh scattering peak at 470 nm was enhanced considerably and the fluorescence peak at 350 nm was quenched. The Rayleigh scattering spetra and TEM observation revealed that the formation of HSA-SiW nanoparticles and interface formation are main factors that causes the fluorescence quenching and Rayleigh scattering enhancement.