脂肪酶酶粉催化的无溶剂酯交换反应

Solvent-free Transesterification Catalyzed by Lipase Powder

用硅胶等作为载体 ,以癸酸乙酯与己醇的酯交换作为模型反应 ,将脂肪酶酶粉、底物直接与硅胶等混合 ,进行无溶剂酶催化酯交换反应。结果表明 ,体系中水的含量、硅胶用量以及反应时间均对酯交换反应有较大影响。当硅胶用量 2 5mg ,Rhizopusarrbizus脂肪酶酶粉 5mg ,加水量 (pH =7 0 ,5 0mmol/L磷酸缓冲液 )18μL,Candidarugosa脂肪酶酶粉 10mg ,加水量 15 μL时 ,2种脂肪酶催化酯交换反应的速度显著增加 ,反应 2 0h后 ,转化率可以达到 90 %以上。与硅胶混合的脂肪酶可以反复使用多次。

The solvent-free transesterification of ethyl decanoate with 1-hexanol has been carried out by cata- lysis through direct mixing of powdered lipase, Rhizopus arrbizus or Candida rugosa, with carrier and substrates. The results showed that water content, amount of silica gel and reaction time affected the rate or yield of the reaction. For a mixture of 25 mg of silica gel, 5 mg of Rhizopus arrbizus lipase powder, 18 μL of 50 mmol/L phosphate buffer(pH=7.0) or 10 mg of Candida rugosa lipase powder, 15 μL of buffer, the both lipases catalyzed reactions proceed readily, and after 20 h, yield greater than 90% could be achieved. The lipase mixed with silica gel can be reused many times.