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华东地区汉族脂肪分化相关蛋白基因SNP的研究 被引量：3

The SNP Detection of ADRP Gene for East Chinese Hans
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摘要 目的研究华东地区汉族 2型糖尿病病人脂肪分化相关蛋白 (ADRP)基因的单核苷酸多态 (SNP)。方法使用PCR测序方法 ,对ADRP基因的启动子、外显子以及临近的内含子进行测序 ,确定该基因在华东地区汉族2型糖尿病病人中所存在的SNP。　结果所测ADRP基因片段总长度为 5 0 0 3个碱基 ,有 2 1个SNP ,高频、低频分别是 5、16个。　结论ADRP基因在华东地区汉族 2型糖尿病病人中存在SNP ;对某些SNP进行基因分型可以进一步确定ADRP基因中与 2型糖尿病相关的SNP存在情况。 Objective To identify single nucleotide polymorphism (SNP) of ADRP gene in type 2 diabetics of Chinese Hans residing in east and south-east China. Methods Sequences of proximal promoter and coding regions (including sequence near splicing site) of ADRP gene were detected by PCR-sequencing. Results A total of 21 SNPs were identified for the ADRP gene; there were 5 high frequency SNPs and 16 low frequency SNPs. Conclusion SNPs were identified in the ADRP gene from patients of type 2 diabetes in Chinese Hans residing in east and south-east China; these results should be confirmed or rejected in the genotyping study for ADRP gene in the future.
作者 吴刚 李果 骆天红 董艳 黄薇 罗敏
机构地区 上海第二医科大学瑞金医院内分泌代谢病科上海市内分泌研究所 国家人类基因组南方研究中心
出处 《上海第二医科大学学报》 CSCD 2003年第4期294-296,304,共4页 Acta Universitatis Medicinalis Secondae Shanghai
基金 国家 973项目 (G1 9980 51 0 1 7)
关键词 2型糖尿病 脂肪分化相关蛋白 单核苷酸多态性 聚合酶链反应 华东地区 gene single nucleotide polymorphism type 2 diabetes
分类号 R587.1 [医药卫生—内分泌]
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参考文献8

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同被引文献23

  • 1袁中华,杨永宗,尹卫东,易光辉,万载阳.反义adipophilin寡核苷酸降低ACAT活性(英文)[J].生物化学与生物物理进展,2004,31(10):930-935. 被引量：11
  • 2Wang Q, Li H, Li N, Leng L, Wang Y, Tan Z. Identification of single nucleotide polymorphism of adipocyte fatty acid-binding protein gene and its association with fatness traits in the chicken. Poultry Science, 2006, 85: 429-434.
  • 3Wolins NE, Quaynor BK, Skinner JR, Schoenfish MJ, Tzekov A, Bickel PE. S3-12, Adipophilin, and TIP47 package lipid in adipocytes. Journal of Biological Chemistry, 2005, 280(19): 19146-19155.
  • 4Subramanian V, Rothenberg A, Gomez C, Cohen AW, Garcia A, Bhattacharyya S, Shapiro L, Dolios G, Wang R, Lisanti MP, Brasaemle DL. Perilipin A mediates the reversible binding of CGI-58 to lipid droplets in 3T3-L1 adipocytes. Journal of Biological Chemistry, 2004, 279(40): 42062-42071.
  • 5Jun Gao, Ginette Serrero. Adipose differentiation related protein (ADRP) expressed in transfected COS-7 cells selectively stimulates long chain fatty acid uptake. Journal of Biological Chemistry, 1999, 274 (24): 16825-16830.
  • 6Prats C, Donsmark M, Qvortrup K, Londos C, Sztalryd C, Holm C, Galbo H, Ploug T. Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine. Journal of Lipid Research, 2006, 47(11): 2392-2399.
  • 7Jiang H, Serrero G. Isolation and characterization of a full-length cDNA coding for an Adipose Differentiation-Related Protein. Proceedings of the National Academy of Sciences, 1992, 89: 7856.
  • 8Dicker A, Astrom G, Sjolin E, Hauner H, Arner P, Harmelen V. The influence of preadipocyte differentiation capacity on lipolysis in human mature adipocytes. Hormone and Metabolic Research, 2007, 39(4): 282-287.
  • 9Wang Z, Jiang T, Li J, Proctor G, McManaman JL, Lucia S, Chua S, Levi M. Regulation of renal lipid metabolism, lipid accumulation, and glomerulosclerosis in FVBdb/db mice with type 2 diabetes. Diabetes, 2005, 54(8): 2328-2335.
  • 10Kim TH, Choi BH, Chang GW, Lee KT, Lee HY, Lee JH, Kim KS, Park CK, Moran C. Molecular characterization and chromosomal mapping of porcine adipose differentiation-related protein (ADRP). Journal of Animal Breeding and Genetics, 2005, 122(4): 240-246.

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上海第二医科大学学报
2003年 第4期

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