PDGF-BB对肾小球系膜细胞[Ca^(2+)]_i和细胞增殖的影响

Effects of PDGF-BB on [Ca^(2+)]_i and Proliferation in Cultured Glomerular Mesangial Cells

目的研究肾小球系膜细胞 [Ca2 + ]i的变化与系膜细胞增殖之间的关系。 方法以体外培养的系膜细胞为研究对象 ,激光共聚焦显微术结合Fura- 3染色法 ,动态测定细胞 [Ca2 + ]i,MTT法测定细胞增殖。 结果血小板衍生生长因子 (PDGF) -BB可浓度依赖性地引起肾小球系膜细胞 [Ca2 + ]i 的升高 ,并促进细胞增殖。钙通道阻滞剂和血管紧张素转换酶抑制剂均可同时抑制PDGF -BB引起的细胞钙浓度升高和细胞增殖。中药成分雷公藤多甙在显著抑制系膜细胞增殖的同时 ,对细胞游离钙却无影响。 结论肾小球系膜细胞 [Ca2 + ]i 的升高和细胞增殖之间存在着正性关联 ,但 [Ca2 + ]i

Objective To investigate the relationship between [Ca 2+ ] i alteration and proliferation in cultured glomerular mesangial cells. Methods The rat mesangial cells were cultured.[Ca 2+ ] i were measured by confocal laser scanning microscopy and Fura-3 fluorescence dyeing techniques. Cell growth was measured by MTT assay. Results Platelet-derived growth factor (PDGF)-BB could increase [Ca 2+ ] i in a dose-dependent manner, and at the same time promote mesangial cells proliferations. After preincubation with calcium channel blocker nifedipine or angiotensin converting enzyme inhibitor captopril, both the increase of [Ca 2+ ] i and cell proliferations induced by PDGF-BB were inhibited. Tripterigium wilfordii glycosides (TMG) could significantly inhibit mesangial cell proliferation, but had no effect on [Ca 2+ ] i. Conclusion There is a positive relationship between [Ca 2+ ] i elevation and proliferation in glomerular mesangial cells, but the increase of [Ca 2+ ] i is not the only way for proliferation.