玉米P_(25)自交系抗锈病基因的遗传分析及SSR分子标记定位

Studies of Genetic Analysis and SSR Linked Marker Location of Gene Resistance to Southern Rust in Inbred Line P_(25) of Maize

以玉米南方型锈病免疫自交系P2 5和感病自交系F3 4 9及F1、F2 、B1和B2 为材料 ,采用主基因 多基因混合遗传模型研究了P2 5的抗病遗传规律。结果表明 :自交系P2 5的抗病基因为一主基因 ,表现为加性效应 ,没有检测出多基因 ,其在F2 、B1和B2 群体的遗传率分别为 81 88%、38 14 %和 5 5 1%。利用SSR分子标记技术 ,以组合P2 5×F3 4 9的F2 :3 家系作为构图群体 ,构建了玉米SSR遗传连锁图谱 ,并将玉米抗南方型锈病基因定位于 10号染色体上 ,与phi0 5 9标记的遗传距离为 5 8cM。

Using P_ 25 (immune inbred line),F_ 349 (susceptible inbred line) and the derived population F_1?F_2?B_1 and B_2 as materials,we investigated the heredity the of disease resistance gene to maize southern rust ( Puccinia polysora Underw.) through the major-gene and polygene inheritance model. The results indicate that a major resistance gene exists in the inbred line P_ 25 and expresses with additive effect.We didn't find any multi-genes.The inheritabilities of this major resistant gene among F_2,B_1 and B_2 were 81.88%?38.14% and 55.1%,respectively.We constructed a maize SSR linkage map using P_ 25 ×F_ 349 F_ 2:3 population,and located the resistant gene on chromosome 10.The genetic distance between this gene and phi059 marker was 5.8 cM.