三苯氧胺对大鼠乳腺增生形态学的影响

Effect of different dosage of tamoxifen on the morphology of the mammary hyperplasia of rats

目的 :探讨不同剂量的三苯氧胺 (TAM )对实验性乳腺增生症形态学的影响。方法 :用雌二醇和黄体酮诱导Wistar大鼠乳腺增生动物模型。 180只雌性大鼠随机分为六组 :A组 :空白对照组 ,B组 :模型对照 (单用性激素 )组 ,C组 :性激素加TAM (16 6 .7μg)组 ,D组 :性激素加TAM (333μg)组 ,E组 :性激素加TAM(5 0 0 μg)组 ,F组 :性激素加TAM (6 6 7μg)组 ,喂养 2个月后 ,经光镜及电镜观察。结果 :光镜观察 :C组、D组用药前后P >0 .0 5 ,无明显差别。E组、F组P <0 .0 1,用药前后对比有显著差别。说明E组及F组剂量的TAM有明显抑制乳腺增生的作用。结论 :三苯氧胺有抑制雌二醇和黄体酮诱导的Wistar大鼠乳腺增生的作用 ,尤E组及F组明显。

Objective:To study the effect of different dosage of tamoxifen (TAM) on the morphological change of experimental mammary hyperplasia.Methods:The animal model of mammary hyperplasia of Wistar rats was induced with estradiol and progesterone. One hundred and eighty female Wistar rats were selected randomly into group A (control group ) , group B (model control group), group C (166.7 μg dosage of TAM),group D(333μg dosage of TAM) ,group E(500μg dosage of TAM),and group F(667μg dosage of TAM) and fed for 2 months.Samples of breasts were collected and observed under light microscope and electronmicroscope.Results:For light microscope observation :there were obvious difference between premedication and postmedication in group E and F, respectively ( P <0.01),but no obvious difference were found in group C and D( P >0. 05), implying that 500 μg dosage and 667 μg dosage of TAM had obvious inhibitory effect on mammary hyperplasia under light microscope.Conclusion:Through light microscope and electronmicroscope observation,TAM especially 500μg and 667μg dosage proves to have obvious inhibitory effect on mammary hyperplasia induced by estradiol and progesterone.