骨髓间充质干细胞体内诱导分化为心肌细胞的初步实验观察

An experimental study on mesenchymal stem cells differentiated into cardiomyocytes in vivo

目的 :初步观察MSCs体内诱导分化为心肌细胞的能力。方法 :从大鼠双侧股骨骨髓获得MSCs ,在体外纯化、扩增后 ,DAPI进行细胞标记 ,然后注射到急性心肌梗塞模型鼠和正常大鼠的心肌组织内 ,饲养 2～ 4周后 ,处死动物在注射点获取心肌标本 ,初步采用HE染色和电镜观察形态学的方法对分化的心肌细胞进行鉴定 ,并用免疫组化法从组织学上对转化心肌细胞进行心肌特异性抗原的检测。结果 :MSC进行DAPI的标记效率高 ,电镜观察与宿主心肌细胞间形成了闰盘 ,组化检测有心肌特异性抗原的出现。结论 :在宿主心肌组织内 ,MSCs具有分化为心肌细胞的能力。

Objective:To investigate the ability of mesenchymal stem cells differentiated into cardiomyocytes in vivo.Methods:MSCs isolated form rats bilateral tibias were purified,expanded in vitro culture, labeled with DAPI, then injected into the myocardium of the recipients(including acute myocardial infraction and normal rats).The hearts were harvested 2～4 weeks after implantation.Morphology of the differentiated cardiomyocytes was identified using HE stain and electron microscopy.Cardiac-specific antigen was detected using immunohistochemistry.Results:Viable cells labeled with DAPI can be identified in host myocardium at all time points after implantation.Implanted MSCs showed the growth potential in myocardial environments, after 4 weeks, donor cells derived from MSCs demonstrated myogenic differentiation with the expressing of myosin heavy chain.Donor cells demonstrated cardiomyocytes phenotypes with the expressing of cardiac-specific antigen Cx43.Meanwhile,electron microscopy examination demonstrated the existence of intercalated disks between donor cells and host cells.Conclusion:MSCs can differentiate into cardiomyoytes in host myocardial microenvironments.