胶原构成与不同年龄增生性瘢痕形成的研究

A study on collagen constitute and formation of hypertrophic scar in different age periods

目的 :通过观察不同年龄正常皮肤 (NS)、增生性瘢痕 (HTS)胶原构成、比例 ,初步探讨各年龄阶段HTS形成的可能机理及条件。方法 :采用SABC免疫组织化学方法观察胎儿、1～ 19岁 (儿童青少年组 )、2 0～ 5 0岁 (成年组 )、>6 0岁 (老年组 )正常皮肤和 1～ 19岁、2 0～ 5 0岁HTS中Ⅰ、Ⅲ型胶原含量和比例。结果 :①胎儿组Ⅲ型胶原含量高于Ⅰ型胶原 ,其余组为Ⅰ型胶原高于Ⅲ型胶原 ;正常皮肤Ⅰ /Ⅲ型胶原比例随年龄增加呈上升趋势。HTS组织的Ⅰ /Ⅲ型胶原比例明显高于正常皮肤(NS) ,1～ 19岁HTS组Ⅰ /Ⅲ型胶原比例较 2 0～ 5 0岁HTS组增高 ,平均为 6 .4 8,3.76 ;HTS组各病程之间Ⅰ /Ⅲ型胶原比例无差异。结论 :Ⅰ、Ⅲ型胶原的含量和比例决定着皮肤的正常结构 ,Ⅰ ,Ⅲ型胶原比例的失调与不同年龄HTS的形成有着密切关系。

Objective:To investigate the mechanism of hypertrophic scar formation in different age periods.Methods:The expression of type I and type Ⅲ collagen of hypertrophic scar in different age periods was measured with immunohistochemistry which was divided into two groups 1～19 years old and 20～50 years old,and the same measure was adopted in normal skin divided into four groups-fetal,1～19,20～50,and more than 60 years old .The quantities of type I and type Ⅲ collagen and the ratios were analyzed.Results:①The ratio of type Ⅰ/type Ⅲcollagen of normal skin had a guadually increasing tendency.②The ratio of type Ⅰ/type Ⅲ collagen of HTS was increasing compared with normal skin(NS),and relative quantities of type I collagen of HTS was significantly increasing.The ratio of type Ⅰ/type Ⅲcollagen of HTS in group of 1～19 year old was highter than that in the group of 20～50 year old .Conclusion:Normal structure of the skin is decided by quantities and ratio of type I and type Ⅲ collagen.The formation of hypertrophic scar in different age periods relates to maladjustment of the ratio of type I and type Ⅲ collagen.Collagen synthesis and regulation of its constitute are finished after about six months.They keep relatively stable within 6 month to 2～3 years.