正常兔肝内微循环血管铸型观察

The study of hepatic microcirculation in normal rabbits

目的 :观察正常兔肝内三维微循环血管的分布。方法 :应用甲基丙稀酸甲酯 (Methylmethaorylate)对兔肝动脉 (HA)和门静脉 (PV)进行铸型 ,再用扫描电子显微镜观察肝内微循环分布。结果 :家兔肝窦状间隙直径大致范围在 9.5～ 10 .9μm之间 ,HA/PV ,HA/BD和PV/BD 3者在 2～ 4级分叉范围内分别为 0 .2 8± 0 .0 3、0 .2 6± 0 .0 6和 0 .89± 0 .12 ,肝血窦 /肝细胞索两者面积比值为 31.87% ,而出入肝血液的各流变学指标测定没有变化 (P >0 .0 5 )。结论 :应用甲基丙稀酸甲酯铸型和扫描电子显微镜 ,可以较好的观察兔肝内微循环的分布 ,为研究病理情况下肝内微循环的变化作良好的对比研究。

Objective:To study three-dimensional hepatic microcirculation in normal rabbits.Methods:The corrosion casts was produced using methyl methacrylate and the HA,PV and BD were observed by scanning electron microscopy(SEM).Results:The diameter range of hepatic sinus was about 9.5～10.9μm,the ratio of HA/PV,HA/BD and PV/BD were about 0.28±0.03,0.26±0.06 and 0.89±0.12.The ratio of hepatic sinus and cells was about 31.87%.There was no significant rheological respectively between entering and outflowing liver blood.Conclusion:It is an effective method to study the hepatic microcirculation by using both methylmethacrylate and scanning electron microscopy.The results can serve as contrast standard for hepatic pathological research.