兔骨膜成骨细胞体外培养体系的建立

Department of a in Vitro Culture System of Osteoblasts Isolated from Juvenile Rabbit Periosteum

本文用兔骨膜成骨细胞分离培养建立了成骨细胞生物学模型。本法有骨膜取材方便、骨膜生发层所含的成骨细胞量多而集聚的优点,分离培养技术也简单易行,为研究成骨细胞的代谢及成骨潜能提供了有用的工具。

We have developed a in vitro culture system of osteoblasts isolated from juvenile rabbit periosteum. The cultivation could be served as an osteoblast biological model. The newly developed method has the following advantages: (a) The periosteum is easy to obtain. (b) The periosteal cambium layer contains a great deal of osteoblasts. (c) The procedure of isolating osteoblasts from periosteum is less complex than that from bone tissue. The cultivation could be regarded as a useful technique for studying the metabolism and osteogenic capacity of osteoblasts in vitro.