HPLC同步测定肌酸、磷酸肌酸、腺苷酸及次黄嘌呤类物质

Synchronous Determination of Creatine Compounds, Adenine Nucleotides, Hypoxanthine and Inosine with HPLC

本文用反相HPLC法,以TBA(四丁基氢氧化铵)作离子对试剂,用含13％甲醇的磷酸钾缓冲液进行等比洗脱,同步分离测定了肌酸(Cr)、磷酸肌酸(Crp)、次黄嘌呤(HX)、次黄嘌呤核苷(INO)及AMP、ADP、ATP七种标准品的混合物。结果表明,在210nm波长下,10分钟内上述七种物质均完全分开且基线未漂移,重现性好,是一种简便、快速和可靠的方法。

Synchronous determination of creatine Compounds, adenine nucleotides, hypoxanthine and inosine with reversed—phase HPLC was studied. The distinct separation was achieved within 10 minutes at 210nm using radial—PAK C_(18) Cartridge column under an isocratic elution of 220mmol/L K_2HPO_4/KH_2PO_4, mmol/L TBA and 13% methanol. This method proved to be reproducible, simple and accurate.