早期效应细胞因子对脐血AC133^+细胞表面趋化因子受体和粘附分子的调节作用

Regulation of early-effective cytokines on the expression of chemokine receptors and adhesion molecules in cord blood AC133^+ cells

目的 探讨不同细胞因子组合对脐血AC133+ 细胞表面趋化因子受体 (CXCR4 )和粘附分子VLA4 (CD4 9d)表达的影响。方法 采用双色直接免疫荧光标记法 ,使用流式细胞仪测定新鲜分离的、不同培养条件下体外培养 7d的脐血AC133+ 细胞表面CXCR4和VLA4 (CD4 9d)的表达情况。结果 2 6例新鲜脐血AC133+ 细胞中CXCR4的表达率为 (46 .3± 11.5 ) % ;VLA4的表达率为(5 1.2± 17.3) %。在无血清、有细胞因子存在的条件下 ,短期液体扩增培养后 ,脐血AC133+ 细胞中CXCR4和VLA4的表达率发生变化 ,其中在早期效应细胞因子SCF、FL和TPO组合下 ,CXCR4和VLA4表达率为 (6 8.9± 15 .8) %和 (73.6± 19.8) % ,与新鲜脐血比较 ,差异非常显著 ,P <0 .0 1。结论 在体外短期扩增培养过程中 ,早期效应细胞因子能显著上调脐血AC133+ 细胞CXCR4和VLA4的表达 ;用SCF、FL和TPO组合扩增脐血 ,不仅能获得足够数量的造血干 /祖细胞 ,还能增加造血干 /祖细胞的迁移和归巢能力。

Objective To explore the influence of cytokine combinations on the expression of chemokine receptors (CXCR4) and adhesion molecule VLA4 (CD49?d) in cord blood AC133 + cells. Method The expression of CXCR4 and VLA4 in fresh CB AC133 + cells cultured for 7 days were analyzed by flow cytometric two-color director immunoflurescence methods. Results In 26 fresh CB, the positive rate of CXCR4 and VLA4 was ( 46.3 ± 11.5 )% and ( 51.2 ± 17.3 )% respectively. After short-term incubation with cytokines in vitro, the expression rate of CXCR4 and VLA4 changed. The expression of them was especially higher in the presence of SCF, FL and TPO combinations. Conclusions As well as CB CD34 + cells, CXCR4 and VLA4 are expressed in CB AC133 + cells. During short-term culture in vitro, the early-effective cytokines can up-regulate the expression of CXCR4 and VLA4. In the CB expansion system, SCF, FL and TPO combinations may generate enough hematopoietic stem/progenitor cells while contain suitable migration and homing capabilities.