染发剂对家兔潜在性致癌作用的实验研究

Potential Carcinogenic Effect of S-brand Hair Color Cream on Rabbit

目的 研究S牌染发剂对家兔的潜在性致癌作用。方法 健康家兔经皮涂敷S牌染发剂 6个月(每周涂抹 2次 ) ,解剖动物 ,取内脏组织作免疫组织化学及细胞周期分析。结果 免疫组织化学检测显示 ,肝、脾、胸腺各组织中p5 3蛋白、增殖细胞核抗原、及bcl 2癌基因蛋白均呈不同程度的阳性反应。细胞周期分析显示 ,染发剂组家兔肝、脾、胸腺 3种组织除了二倍体细胞群外 ,还有 1组异倍体细胞群。结论 S牌染发剂对家兔可能具有潜在的致癌作用。

Objective To study the potential carcinogenic effect of S brand hair color cream on rabbit.Methods S brand hair color cream was pasted on the skin of rabbits for six months(twice a week).Rabbits were dissected,and their liver,spleen and thymus were prepared for immunohistochemical and cell cycle analyses.Results Immunohistochemical analysis showed positive effect for p53 in all three tissues of liver,spleen and thymus,which were also positive for both proliferating cell nuclear antigen(PCNA)and bcl 2 onco protein.Aneuploid was found in all three tissues besides normal diploid by cell cycle analysis.Conclusions S brand hair color cream might be a potential carcinogen.