分化抗原gp42表达对B细胞株增殖和血清撤除致死的影响

Effect of differentiation antigen 5D4 expression on proliferation and serum deprivation-induced death of 3D5 cell

目的 研究分化抗原gp4 2表达对 3D5B细胞株功能的影响。方法 以生长曲线检测细胞的增殖 ,碘化丙啶 (PI)染色 ,DNA流式细胞仪分析细胞周期 ,Annexin Ⅴ PI染色检测细胞死亡 ,间接免疫荧光染色流式细胞仪分析CD分子的表达 ,用Fluo 3AM作探针激光共聚焦显微镜观察胞内Ca2 + 浓度的变化。结果 分化抗原gp4 2表达抑制的 3D5细胞生长明显加速 (P <0 .0 0 1) ,从S期进入G2 M期的比率明显增加。对血清撤除所致的死亡发生抵抗。分化抗原gp4 2表达抑制的 3D5细胞间的粘附消失 ,CD11c的表达明显减弱。在McAb 5D4的刺激下 ,3D5细胞胞内的Ca2 + 浓度随时间逐步增高。结论 分化抗原gp4 2表达抑制致使 3D5细胞生长加速 ,细胞间粘附消失。分化抗原gp4 2是一个信号传导分子。

Objective To investigate the effect of differentiation antigen gp42 expression on 3D5 cell function. Methods Cell proliferation was assayed by drawing of cell growth curves. Cell cycling was detected by propidium iodine (PI) stainning and flowcytometry. Measurement of CD molecule expression was carried out by means of indirect immunofluorescence staining and flowcytometry. Change of intracellular Ca 2+ concentration was measured by a laser confocal microscopy using Fluo-3AM as a probe. Results Growth of the cell with an inhibition of differentiation antigen gp42 expression was greatly enhanced (P<0.001) accompanied by entrance of more cells into G2 phase. The cells with an inhibition of differentiation antigen gp42 expression became resistant to death resulted from serum deprivation. The adhesion among these cells was profoundly reduced with reduction of CD11c expression. Being stimulated by McAb 5D4, 3D5 cell showed an increase of intracellular Ca 2+ concentration. Conclusion Inhibition of differentiation antigen gp42 expression results in an enhancement of growth of 3D5 cells and a pronounced reduction of adhesion among the cells. Differentiation antigen gp42 is a protein molecule with signaling function.