邻苯二甲酸二-(2-乙基己基)酯对小鼠胚胎发育毒性的体外实验研究

Effects of Di(2-ethylhexyl) phthalate(DEHP) on mouse embryos development in vitro

为探讨邻苯二甲酸 二 2 乙基己基酯 (Di 2 ethylhexylphthalate ,DEHP)对体外培养小鼠胚胎的发育毒性 ,采用植入后全胚胎培养模型 ,将 8.5天龄Balb c小鼠胚胎移入含DEHP即刻离心血清培养 48h ,DEHP终浓度为 0、12 .5、2 5、5 0、10 0和 2 0 0mg L ,观察DEHP对小鼠胚胎生长发育和组织器官形态分化的影响。结果显示DEHP对体外培养小鼠胚胎生长发育毒性的最大无作用剂量为 12 .5mg L ,≥ 2 5mg L的DEHP可诱发胚胎生长迟缓及组织器官形态分化异常 ,出现心脏、神经系统、腮弓发育异常及小肢芽、体位异常等畸形 ;10 0mg L的DEHP对体外培养胚胎偶尔呈致死效应 ;2 0 0mg L时各组织器官形态分化均出现了异常 ,但未发现死胎。上述改变具有明显的剂量 -效应及剂量 -反应关系。提示DEHP对体外培养的小鼠胚胎具有胚胎毒性 。

Whole-embryo culture was used as the model system to study the effects of Di(2-ethylhexyl) phthalate (DEHP) on mice embryonic development. Mouse embryos (8.5 days old) during the period of organogenesis were exposed to immediately centrifugal serum (ICS) with DEHP at concentrations of: 0,12.5, 25,50,100,200 mg/L for 48 hours. The results showed that DEHP could cause damage to embryonic development and there was a significant dose-response and dose-effect relationship between concentration of DEHP and its embryonic developmental toxicity. At higher levels ((25 mg/L),DEHP could induce embryonic developmental retardation and significant morphological abnormalities. Abnormal heart, neural system, arch and small limb-bud development were the most common gross morphological abnormalities. But there was no significant increase in the number of nonviable embryos. The finding suggested that DEHP can induce developmental toxicity in vitro at higher level and teratogenicity may be the major effect.