木瓜粉对体外培养神经细胞缺氧损伤治疗效果的形态学观察

Morphologic observation of papaya on anoxic damaged cerebral neurocytes of therapeutic efficacy in vitro

为观察木瓜粉对体外培养鼠胚大脑神经细胞缺氧损伤治疗效果的形态学影响 ,采用无血清体外细胞培养方法 ,培养鼠胚大脑神经细胞 ,将神经细胞置于缺氧环境造成缺氧损伤 ,然后加入含木瓜粉的培养液 ,将细胞分为 4组 ,Ⅰ组、Ⅱ组、Ⅲ组为缺氧组 ,其木瓜粉浓度分别为 0、0 1和 0 5mg ml;IV组为非缺氧未加木瓜粉组。于细胞培养后的第 4天收集细胞 ,进行有关指标的观察、测定。结果 :添加木瓜粉的II组MTT高于其它 3组 ,并有显著性差异 (P <0 0 5 ) ,各组神经细胞TCHE之间也存在显著性差异 (P <0 0 1) ,其中Ⅱ、Ⅲ神经细胞TCHE活性高于Ⅰ、Ⅳ组 (P <0 0 5 ) ,Ⅲ组神经细胞TCHE活性高于Ⅱ组 (P <0 0 5 )。形态观察显示 ,Ⅰ组神经细胞数量少 ,外观残破 ,界限不清 ,突起较细疏短缺 ,可见凋亡小体 ;添加木瓜粉的Ⅱ、Ⅲ组多数细胞铺片良好 ,形态丰满 ,界限清楚 ,突起较粗壮 ,突起间网络比较稠密 ,核染色质虽有些凝聚 ,但未见凋亡小体。结论

Objective: To observe the effects of papaya on the therapeutic efficacy of cerebral neurocytes following anoxic damage in vitro. Methods: The rat embryonic cerebral neurocytes were cultured with serum-free medium. Papapa was added in while anoxic damage occurred and the neurocytes were divided into four groups (group I-anoxic damage 0mg/ml, group II-anoxic damage 0.1mg/ml, group III-anoxic damage 0.5mg/ml and group-IV non-anoxic damage 0mg/ml). At the end of the experiment, cultured neurocytes were collected and biochemical indexes were detected and, a pathological observation was made by using electronic microscope. Results: The activity of MTT in group II was significantly higher than that in the other three groups(P<0.01). Compared with group I and IV, the level of TCHE in group Ⅱ and Ⅲ was increased significantly(P<0.05), and the level of TCHE in group Ⅲ was much higher than that in group Ⅱ (P<0.05).The morphological observation showed that the number of neurocytes in group I decreased, and the appearance of cell was broken, the outer limits were not clear, apophysises were thin and short, and apoptosis body can be found. In group II and Ⅲ, stretched preparation of majority cells was good, the bodies of neurocytes was full-grown, the outer limits were clear, apophysises were strong and network was dense, chromatin was partly condense, but apoptosis bodies can not be found. Conclusion: Papaya can promote the recovery of anoxic damage of rat cerebral neurocytes in structure.