bcrabl融合基因对β1整合素和L-选择素基因表达的影响

Effects of bcr/abl fusion gene on expression of β1 integrin and L-selectin in mouse chronic myeloid leukemia cells

目的 研究bcr abl融合基因对 β1整合素和L 选择素表达的影响。 方法 应用半定量RT PCR方法检测bcr abl融合基因阴性和阳性的慢性髓系白血病 (CML)细胞中 β1整合素和L 选择素mRNA的表达水平。同时观察酪氨酸激酶抑制剂Rb C Box基因转染bcr abl基因阳性细胞后 β1整合素和L 选择素mRNA表达水平的变化。结果 在bcr abl基因阳性细胞中 ,L 选择素mRNA表达水平明显低于bcr abl阴性细胞 ,转入Rb C Box基因后 ,L 选择素mRNA的表达水平与bcr abl阴性细胞相似。bcr abl阴性和阳性细胞及转染Rb C Box基因细胞中 β1整合素mRNA表达水平差异均无显著性。 结论 bcr abl融合基因可抑制CML细胞中L 选择素mRNA的表达 ,对 β1整合素mRNA的表达影响较小。

Objectives To explore the effects of p210 bcr/abl fusion gene on expression of β1 integrin and L selectin mRNAs in mouse chronic myeloid leukemia(CML) cells. Methods Comparisons of β1 integrin and L selectin mRNA levels among p210 bcr/abl negative, p210 bcr/abl positive, and p210 bcr/abl Rb C Box positive cells were undertaken by quantity RT PCR. Results In p210 bcr/abl positive cells, L selectin mRNA level was decreased, but β1 integrin mRNA expression had no change as compared to those in p210 bcr/abl negative cells. When inhibition of bcr abl tyrosine kinase activity by Rb C Box, the L selectin mRNA expression restored to normal (similar to p210 bcr/abl negative cells). Conclusion p210 bcr/abl oncoprotein inhibits expression of L selectin mRNA, but not of β1 integrin mRNA.