无创尿硫测定含硫氨基酸的分解代谢

Noninvasive detection of urinary sulfur for measuring the catabolism of sulfur amino acid

目的 验证尿硫测定是否可以在生长迅速的新出生的小猪模型上精确反映含硫氨基酸的分解代谢情况。 方法 在有充分营养支持的小猪肠内营养模型上 ,观测静脉注射的无机硫酸盐、以及蛋氨酸中的硫是否完全经尿排泄。 结果以总硫方式测定的无机硫酸盐和蛋氨酸在尿中的回收率分别为的 95 6 %和 10 5 5 %。 结论 在生长迅速的小猪模型上 ,尿硫测定这一无同位素标记、无创的方法可以精确反映含硫氨基酸的分解代谢情况。

Objective To test whether urinary sulfur excretion can be used as an accurate indicator of the catabolism of sulfur amino acid in growing newborn piglets. Methods Using a well-nourished enteral nutrition piglet model,we tested whether intravenous inorganic sulfate and methionine were fully excreted as sulfur in the urine. Results Recovery rate of inorganic sulfate and methionine as total sulfur in urine were 95.6% and 105.5%, respectively. Conclusions Detection of urinary sulfur, as a non-tracer and noninvasive method, may be employed to accurately measure the catabolism of sulfur amino acid in the growing piglet model.