摘要
目的 探讨CD5 9检测对阵发性睡眠性血红蛋白尿症 (paroxysmalnocturnalhemoglobinuria ,PNH)及再生障碍性贫血 (AA) PNH综合征的临床诊断意义。方法 用FITC标记的CD5 9单抗 ,以流式细胞仪检测 13例PNH、37例AA和 2 0例正常人外周血红细胞和粒细胞膜表面CD5 9的表达情况。结果 正常人红细胞和粒细胞CD5 9表达阳性率均 >98% ;13例PNH患者红细胞及粒细胞CD5 9表达阳性率均 <90 % ;37例再障患者中 2 4例表达正常 ,13例红细胞或粒细胞CD5 9表达阳性率均有不同程度的减低。结论 CD5 9检测是诊断PNH较直接而又特异、敏感的方法 ,特别是对表现不典型的PNH及AA
Objective To explore the clinical significance of detection of CD59 in the diagnosis of PNH and AA PNH syndrome. Methods The expression of CD59 was detected in RBCs and polymorphonuclear neutrophils (PMNs) from peripheral blood of 13 PNH (including 2 cases of AA PNH syndrome ) and 37 AA patients by flow cytometry along with FITC labelled anti CD59 monoclonal antibody. Results The positive rates of CD59 expression were constantly more than 98% in RBCs and PMNs from normal individuals,whereas less than 90% in RBCs and PMNs from the 13 PNH patients. Of the 37 AA patients, the positive rates of CD59 expression were normal in RBCs and PMNs from 24 cases but decreased to a greater or lesser degree in RBCs or PMNs from 13 cases compared with that in normal individuals. Conclusion Determination of CD59 in peripheral RBCs and PMNs could be considered a direct, specific and sensitive method for the diagnosis of PNH, especially in PNH with atypical manifestations and AA PNH syndrome.
出处
《临床输血与检验》
CAS
2003年第3期176-178,共3页
Journal of Clinical Transfusion and Laboratory Medicine
基金
20 0 1年安徽省优秀青年科技基金资助