针刺对糖尿病大鼠坐骨神经过度非酶性糖基化的调节

Regulatory Effect of Acupuncture on the Abnormality in Non-enzymatic Glycosylation of Neural Proteins in Diabetic Rats

目的 神经蛋白的非酶性糖基化水平异常升高是糖尿病神经病变的重要病理机制之一 ,本文旨在探讨针刺防治糖尿病神经病变的疗效是否与改善坐骨神经蛋白非酶性糖基化水平有关。方法 将腹腔注射链脲佐菌素造成血糖高于 16.7m m ol/ L 的糖尿病大鼠分为模型对照组、针刺治疗组和胰岛素治疗组 ,另设正常组 ,每组 6-7只。造模四周末开始治疗 ,针刺组隔日治疗 1次 ;胰岛素组每天皮下注射胰岛素 ( 4U/ 10 0 g) ,共治疗 2个月。其间动态监测血糖 ,并采用竞争性 AGE EL ISA法测定坐骨神经中 AGEs含量。结果 在造模 6周末 ,针刺组和胰岛素组大鼠的空腹血糖水平比模型组略有降低 ,没有统计学意义 ,而在 12星期末均比模型组明显降低 ( P<0 .0 5 ) ;模型组坐骨神经 A GEs水平为 0 .34 6± 0 .0 5 9U/ ml,比正常组( 0 .197± 0 .0 2 7U/ ml)明显升高 ,而针刺组 ( 0 .170± 0 .0 2 7U/ m l)和胰岛素组 ( 0 .194± 0 .0 34 U/ ml)均比模型组明显降低 ( P<0 .0 5 ) ,与正常组相比无统计学差异 ( P>0 .0 5 )。结论 在糖尿病的早期给与针刺或胰岛素治疗可以防止坐骨神经 AGEs含量的异常增加和积聚 ,使之接近正常水平。说明电针可以通过遏制 AGEs的形成和积聚 。

Objective Abnormality in non-enzymatic glycosylation of neural proteins is thought to impair axonal transport, which may induce diabetic neuropathy. In this paper whether the therapeutic effect of acupuncture on diabetic neuropathy is partially by regulating the formation and accumulation of advanced glycation end products (AGEs) in diabetic rats was explored. Methods The streptozotocin (STZ)- induced diabetic rats with hyperglycemia (>16.7 mmol/L) were divided into diabetes mellitus control group (DM), acupuncture group (Acup) and insulin treated group (Ins). In addition a non-diabetic control group (ND) was used in this experiment. Each group includes 6-7 Wistar rats. The treatment was given one month after the intraperitoneal administration of STZ in a dose of 55 mg/1 kg body weight and lasted for two months. In Acup group the rats were treated with acupuncture once every other day and in Ins group the rats were treated daily with subcutaneous injection of insulin. Fasting blood glucose was measured at the end of the 1st, 6th and 12th week. The concentration of AGEs in the sciatic nerves was measured by the competitive AGEs-ELASA method at the end of 12th week.Results The fasting blood glucose in Acup group and Ins group were lower than that in DM group insignificantly (P>0.05) by the end of the 6th week, however it was significantly at the end of the 12th week (P<0.05, respectively). The concentration of AGEs in sciatic nerve of DM group was significantly higher than that in ND group, (0.346±0.060 U/ml vs 0.197±0.022 U/ml, P<0.05). While in Acup and Ins groups AGEs were (0.170±0.027 U/ml and 0.194±0.034 U/ml, respectively) much lower than that in DM group (P<0.05), no statistical difference between two groups (P>0.05).Conclusion Treatment with acupuncture or insulin can effectively prevent the elevation of AGEs to the normal level in sciatic nerve of STZ-induced diabetic rats. The therapeutic effect of acupuncture on diabetic neuropathy is partially by preventing and delaying the formation and accumulation of AGEs.