对氨基苯甲酸对牙龈卟啉单胞菌细胞形态影响的扫描电镜观察

Scanning Electron Microscope Observation of Morphological Influence P-aminobenzoic Acid (PABA) on Porphyromonas gingivalis

目的 观察不同浓度对氨基苯甲酸 (PABA)对牙龈卟啉单胞菌 (P .gingivalis)菌体形态和黏附能力的影响 ,从而探讨血链球菌 (S .sanguis)在龈下菌斑微生态平衡中的作用。方法 用 1 2浓度的BHI培养基为实验培养基 ,分别加入不同质量浓度的PABA ,对P .gingivalis进行常规培养 ,并对培养出的菌细胞做扫描电镜观察。结果 过高或过低质量浓度的PABA均影响P .gingivalis的菌体形态 ,并对其黏附产生影响。结论 S .sanguis产生的PABA影响P .gingivalis的菌体形态和黏附能力 ,提示S .sanguis对龈下菌斑的微生态平衡具调节作用。

Objective In order to investigate the effect of PABA produced by Streptococcus sanguis on microecological balance of subgingival plaque, different concentrations of PABA were applied to see if it can influence the form and adherence of P.gingivalis.Methods After adding different concentrations of PABA into 1/2 concentration of BHI media, an anaerobic technique was used to culture P.gingivalis. P.gingivalis grew in the medium was observed by a scanning electron microscope.Results Excessively high or low PABA concentration could influence the form and adherence of P.gingivalis. Conclusion PABA produced by Streptococcus sanguis can affect the form and adherence of P.gingivalis.It indicates that Streptococcus sanguis plays regulative effect on the microecological balance of subgingival plaques.