广西番茄灰霉病菌的多重抗药性检测

Multiple Fungidde-resistance of Tomato Botrytis cinerea in Guangxi

进行259个灰霉病菌(Botrytis cinerea)菌株对多菌灵(carbendazim)、腐霉利(procymidone)和乙霉威(diethofencarb)的抗性检测,结果分为6种抗性类型:单抗多菌灵的类型(B^RN^SD^S)占36.3％,单抗乙霉威的类型(B^SN^RD^S)占24.7％,抗多菌灵和腐霉利的类型(B^RN^SD^R)占20.1％,抗多菌灵和乙霉威的类型(B^RN^RD^S)占6.9％,抗腐霉利和乙霉威的类型(B^SN^RD^R)占5.8％,同时抗3种药剂的类型(B^RN^RD^R)占6.2％。各地双抗或多抗菌株出现的频率分别为田阳73.9％,田东61.9％,武鸣30.1％,南宁8.7％,柳州2.6％。大多数多菌灵抗性菌株的抗性水平很高(EC_(50)>1000 mg·L^(-1)),大多数腐霉利抗性菌株的抗性水平较低(EC_(50)<10 mg·L^(-1)),而乙霉威抗性菌株中,双抗或多抗菌株的抗性水平较低(EC_(50)<5 mg·L^(-1));单抗菌株的抗性水平较高(25.1884 mg·L^(-1)<EC_(50)<119.9776 mg·L^(-1))。

Multiple fungicide resistance of 259 Botrytis cinerea isolates collected from five sites in Gaungxi was detected to carbendazim ( B ) , procymidone ( D) and diethofencarb ( N). Resistance can be classified into six types according to being resistant (R) or sensitive (S). Among these BHNSDS type was 36. 3% ,the following were BSNRDS 24.7 % , BRNSDR20.1 %,BRNRDS6.9 % ,BSNRDR 5. 8% ,and BRNRDR was 6. 2%. The frequency of the isolates with multiple fungicide-resistance was 73.9 % in Hanyang,61.9 % in Tiandong,30.1 % in Wuming,8.7 % in Nanning and 2.6 % in Liuzhou respectively. High level resistance to carbendazim was shown in most carbendazim-resistant isolates ( EC50 < 1 000 mg·L-1) ,and low level resistance to procymidone in most procymidone-resistant isolates (EC50 < 10 mg·L-1). However, the diethofencarb resistance isolates showed different resistance level to diethofencarb, high level in the type of BSNRDS (25.188 4 mg·L-1 <EC50 < 119.977 6 mg · L-1) but low level in olhers(EC50 <5 mg · L-1).