摘要
目的:通过检测P21基因表达,以探讨其在大肠癌的发生、发展中的作用及与大肠癌临床病理特征之间的关系。方法:采用免疫组织化学S-P方法,对大肠癌组织(20例)、癌切缘组织(20例)、大肠腺瘤(10例)、炎性息肉(20例)、炎性溃疡(10例)、血吸虫病(6例)及正常大肠粘膜组织(10例)同步检测P21基因蛋白。结果:P21蛋白除了正常大肠粘膜中无阳性表达外,在癌组织、癌切缘组织、腺瘤、炎性息肉、炎性溃疡及血吸虫病肠粘膜组织中表达的阳性率分别为60.00％、10.00％、40.00％、15.00％、10.00％、16.67％。癌组织阳性率与其他组织阳性率相比差异有显著性(P<0.05);P21阳性表达与大肠癌病变部位、患者的年龄和DUKE’S分期均无关(P>0.05),但高、中分化腺癌合并后阳性率与低分化及粘液腺癌合并后阳性率相比差异有显著性(P<0.05)。结论:P21基因表达在大肠癌的发生、发展中起到重要作用。
Objective:To study the role of expression of oncoprotein P21 in the genesis and development of colorectal cancer and the relationship between the expression of oncoprotein and clinical pathological features.Method: Expression of P21of oncoprotein was detected by the immunohistochemistry (S- P method) in 20 cases of colorectal cancer,20 cases of edge tissue of colorectal cancer, 10 cases of colorectal adenoma,20 cases of phlogistic polipus, 10 cases of colorectal inflammatory ulcer,6 cases of schistosmiasis and 10 cases of normal colorectal mucosa.Results:P21 expression was not detected in normal cases and its positive rates were 60.00% , 10.00% , 40.00% , 15. 00% , 10.00% and 16.67% in cases of colorectal cancer, edge tissue of colorectal cancer, adenoma, phlogistic polipus, inflammatory ulcer and schistosmiasis respectively, a remarked difference was existed between cases of cancer and others( P < 0.05). P21 expression was not related to cancer site,age and Dukes stages(P> 0.05). There was a marked difference of positive rate of combined expression between cases of well and mild differentiated cancer and cases of poorly differentiated and mucous adenocarcinoma( P < 0.05). Conclusion: P21 plays an important roles in the genesis and development of colorectal cancer.
出处
《九江医学》
CAS
2003年第2期63-65,共3页
Jiujiang Medical Journal
