大鼠高原严重烧伤后早期肝脏损伤的病理变化及复方红景天参芪花粉对其保护作用

PROTECTIVE EFFECT OF RACC ON THE EARLY PATHOLOGICAL CHANGES OF LIVER IN RATS WITH SEVERE BURN AT HIGH ALTITUDE

目的 :探讨高海拔地区 (海拔 3 480m)大鼠严重烧伤后 (3 0 %Ⅲ度 )肝脏病理变化以及复方红景天参芪花粉对其影响 ;方法 :建立烧伤模型 ,1 56只大鼠随机分为烧伤试验组 (72只 ) ,烧伤对照组 (72只 ) ,正常组 (1 2只 ) ,试验组伤前灌胃给药 ,其余给盐水 ,伤后 3h、6h、1 2h、2 4h、48h和 72h6个时相点剖腹取肝脏 ,1 0 %福尔马林固定 ,常规石蜡包埋切片 ,HE染色 ;结果 :烧伤对照组伤后 3h肝细胞即出现肿胀 ,6h～ 2 4h损害继续加重 ,并出现部分肝小叶坏死 ,可累及整小叶 ,这种严重的器质性损伤在 48h～ 72h仍然持续 ;烧伤试验组 :烧伤后 3h肝细胞轻度肿胀 ,6h～ 2 4h极少出现肝小叶坏死 ,伤后 48h～ 72h肝小叶仅见中央脂肪变性范围较小 ,而累及整小叶及小叶中央静脉周围者少见 ;结论 :复方红景天参芪花粉对大鼠高原严重烧伤后的肝损害有显著的保护作用。

Objective:To study the early pathological changes and the protect effective of Rhadiola Astragalus Codonopsis Compound(RACC) on liver injury in rats with severe burn at high altitude.Method:A total of 156 rats divided randomly into experimental model group (EG.n=72)who were given RACC 4ml by oral before burn and control model group(CG.n=72)who were given saline by oral before burn,normal group as control(NG.n=12).The models of EG and CG were made by fire up to 30% Ⅲ degree burn.The liver of all models were taken at different time after burn and were fixed by 10% formalin.Than the specimens of liver were embedded by paraffin and were made sections with HE dye.Result:In the CG:There was edema of live cells after 3h burn.This injury of liver cells was continual severe and a part of necrosis of liver small leaf and it implicated to the small leaf all of liver happened after 6h～24h burn.This severe organic injure was continual after 48h～72h burn.In the EG:There was light edema of liver cells after 3h burn.The necrosis of liver small leaf were a little after 6h～24h burn.There were small denature area of central fat at small leaf of liver and it was a few of that which implicated to all small leaf of liver and the ceutral of small leaf and the around of veins after 48h～72h burn.Conclusion:There is important protective effect of RACC on liver injur in rats with severe burn at high altitude.