胆囊癌组织中增殖细胞核抗原和癌基因蛋白的表达及相关性研究

Expression of PCNA and c-erbB-2 and relative study in the gallbladder carcinoma

为探索胆囊癌中PCNA和c -erbB - 2表达及其分化、浸润及转移的关系和临床意义。应用HE和免疫组化 (S -P)方法对 98例胆囊癌进行组织学分型、分级、浸润和转移的观察 ,对其中 85例进行PCNA和c -erbB - 2免疫标记。女性 5 7例 ,男性 4 1例。组织学以腺癌为主 6 3例 (占 6 4 3% ) ,免疫组化标记 85例 ,高分化 38例 ,中分化2 7例 ,低分化 2 0例。PCNA阳性表达率为 88 2 % ,其中高表达率在三种分化中分别为 2 9 9%、5 1 9%和 5 5 % ,各组织学分级之间的差异有显著性 (P <0 0 5 ) ;c-erbB - 2阳性率为 36 5 % ,与组织学分级有关 (P <0 0 5 ) ;PCNA高表达的胆囊癌 ,其c-erbB - 2阳性率显著高于低表达者 (P <0 0 5 ) ,PCNA的表达与胆囊癌的组织学分级和转移有关 ;c-erbB - 2的阳性表达与胆囊癌增殖活性、组织学分级和浸润深度有关 ,提示c -erbB -

To study the relationship between the expression of PCNA and c-erbB-2 and differenciation,infiltration and metastasis in gallbladder carcinoma and their clinical signifieance.98 cases of gallbladder carcinoma were studied with HE stain and immunohistochemical makers of monoclonal antibody against PCNA and c-erbB-2(S-P method).Amony 98 cases (female 57 cases,male 41 cases)of gallbladder carcinoma,the tumors in 54 cases were protruded into the tube,44 cases were diffuse,and the tumors were clasted histopathologically as adenocarcinoma (63 cases,64.3%).According to differenciatial degree,the tumore were graded as well differentiation (38 casses) middle (27 cases) and poor (20 cases).The total rate of positive expression of PCNA was 88.2% including high expression 29.9%?51.9% and 55% respectively.Which showed significant difference between the histopathologic grades (P<0.05).The rate of positive expression of c-erbB-2 was 36.5% which was related to histopathlogic grade (P<0.05),The results revealed that high expression of PCNA was associated with the expression of c-erbB-2 (P<0.05).Expression of PCNA was correlated to the histopathologic grade and metastaisis;Positive expression of c-erbB-2 is related to proloferative active and infiltrative depth.The results suggested that the poor prognosis is assotiated with the positive expression of c-erbB-2.