超临界流体萃取吴茱萸中吴茱萸碱和吴茱萸次碱

Study on supercritical fluid extraction of evodiamine and rutaecarpine in Evodia rutacearpa dahurica

目的 :研究超临界流体萃取 (SFE)吴茱萸中吴茱萸碱和吴茱萸次碱的提取工艺 ,并用高效液相色谱法 (HPLC)测定吴茱萸碱和吴茱萸次碱的含量。方法 :采用 95%乙醇作夹带剂 ,运用超临界流体的萃取工艺 ,含量测定时 ,在Water C18柱上 ,以乙腈 水 (43∶57)为流动相 ,流速 :1.0mL·min- 1,检测波长 :2 90nm ,柱温 :40℃。结果 :吴茱萸碱和吴茱萸次碱的标准曲线在0 .0 2 4～ 0 .12 0 μg(r =0 .9994)和 0 .0 2 6～ 0 .13 0 μg(r =0 .9995)之间有较好的线性关系 ;加样平均回收率分别为 :10 2 .7% (RSD= 1.58)和 10 1.7% (RSD =1.79% )。结论 :采用SFE萃取吴茱萸中吴茱萸碱和吴茱萸次碱的提取工艺速度快 ,HPLC法测定吴茱萸碱和吴茱萸次碱的含量精确 。

OBJECTIVE To study the extraction technology of evodiamine and rutaecarpine in Fructus e vodiae by supercritical fluid, extraction and the determination of the content of evodiamine and rutaecarpine by HPLC.METHODS Waters-C 18 column was used with a mobile phase of acetonitrile water(43∶57).The flow rate was 1.0 mL·min -1 .The detection wavelength was set at 290 nm.The column temperature was 40℃.RESULTS The standard curves of evodiamine and rutaecarpine were linear in the ranges fro m 0.024 ～ 1.120 μg(r= 0.9994 )and 0.026 ～ 1.130 μg(r = 0.9995 ).The average recoveries were 101.7% with RSD= 1.79% and 102.7% with RSD= 1.58% .CONCLUSIONS The supercritical fluid extraction is rapid, and the HPLC is accurate and reprod ucible.