白血病骨髓单个核细胞体外培养分泌IL-6,TNF-α的观察

MARROW MONONUCLEAR CELLS OF LEUKEMIA SECRETING IL-6, TNF-α IN CULTURED IN VIT R O

为观察儿童白血病骨髓单个核细胞体外培养上清液白细胞介素 (IL 6) ,肿瘤坏死因子 (TNF α)含量。对 19例儿童白血病骨髓单个核细胞 (PMMNC)体外培养。 9例正常骨髓单个核细胞 (CMMNC)作对照 ,培养 72小时收集上清液 ,用双抗夹心法检测IL 6和TNF α。结果 ,病人及对照骨髓单个核细胞均有自分泌IL 6,TNF α的能力。但CMMNC自分泌IL 6,TNF α的作用明显比PMMNC强 ,P <0 0 5。急性髓性白血病 (AML)与急性淋巴细胞性白血病 (ALL)比较 ,分泌IL 6有差异 ,P <0 0 5 ,但分泌TNF α无差异 ,P >0 0 5。病人血浆IL 6较正常人血浆IL 6含量高。而在病人血浆和正常人血浆中 ,TNF α含量未发现明显差异。结果显示白血病骨髓单个核细胞以及对照单个核细胞均自分泌IL 6,TNF α。植物血凝素 (PHA)诱生IL 6,TNF

Objective: To sudy the content of interleukin 6(IL-6) and tumor necrosis facto r α(TNF-α) in marrow mononuclear cells (MMNC) of children with leukemia(AL) c ultured in vitro. Methods: the samples of MMNC from 19 children with acute leuke mia(PMMNC) and 9 normal controls(CMMNC) were cultured in vitro. Their supernatan t should be gathered after 72 hours at culture. The ELISA method was used to mea sure the IL-6 and TNF-α in the supernatant. Results: Both PMMNC and CMMNC can secrete IL-6 and TNF-α, but the former is weaker than the latter in secreti on( P <0 05). IL-6 levels in acute myeloid leukemia(AML) were higher than those of acute lymphocyte leukemia(ALL)( P <0 05), but the TNF-α levels in both ALL and AML were no difference( P >0 05). The serum IL-6 levels in patient group were significantly higher than those of control group, but serum TNF-α l evels were not diffferent between patient group and control group. Conclusion: M MNC of AL patients and the control group can spontaneously secret IL-6 and TNF -α. The levels of IL-6 and TNF-α secreted in control group are significant higher than those in patient group. There is qualitative defect in MMNC of child ren with acute leukemia as compared with control group, and the reaction to PHA is variable.