多环芳烃化合物菲分解菌的分离鉴定及分解特性研究

Study on Isolation, Identification and Characteristics of Polycyclic Aromatic Hydrocarbons Degrading Bacteria

利用2,3-二羟基联苯喷雾技术,从石油污染土壤中分离到2株菲降解菌。它们可以菲为惟一的碳源和能源良好地生长。在生理生化试验的基础上,利用16S rRNA(小核糖亚单位RNA序列)的方法鉴定这株菌为鞘氨醇单胞菌属细菌。这些菌对菲的降解性是通过在液体培养基内菌体的增加及底物的减少来证实的。静止细胞反应试验表明,这些菌除了菲以外还可降解其它芳烃化合物。

Two bacterial strains have been isolated from petroleum contaminated soils using 2,3-dihydroxybiphenyl spray technique. The isolations were able to use phenan-threne as sole source of carbon and energy. These strains were identified as Sphingomonas species on the basis of biochemical tests and sequences of 16S riboso-mal R.NA genes. Utilization of phenanthrene by the isolates were confirmed by increase in bacterial biom-ass and decrease in substrate concentration in liquid cultures. Resting cells grown in luria broth also showed activity for decomposing other polycyclic aromatic hydrocarbons.