木质素生物合成及其基因调控研究综述

Lignin Biosynthesis and Genetic Regulation

木质素是木材中仅次于纤维素的主要成分 ,占木材干重 1 5 %～ 3 6% ,对造纸业有很重要的影响。通过探索其生物合成途径 ,针对合成过程中的不同酶系 :PAL、C4H、4CL、CCR、CAD、COMT等 ,采用基因工程的反义技术构建调控基因进行转化 ,可以达到降低木质素含量、改变其化学结构和组织的目的。综述了用于木质素基因工程、合成酶基因的克隆和表达研究现状 ,并指出了功能基因组和基因芯片技术在木质素合成途径研究中的应用前景。

Lignin,which plays an important role in pulping industry, represents 15%～36% of dry weight of wood and is a major component second to cellulose. The ways for its biosynthesis are studied and some enzymes such as PAL,C4H,4CL,CCR,CAD and COMT are paid attention to.For the purpose of reduction of the Lignin content and modification of the chemical structure and composition in wood ,genetic engineering of antisense technique has been employed.Here,the research actuality of the engineering,genes cloning of the enzymes and their expression are summarized. This paper also elucidates the bright future of the application of the genomics and gene chip technology in Lignin biosynthesis.