大豆抗胞囊线虫病种质rhg_1和Rhg_4位点的单核苷酸多态性(SNPs)

Single Nucleotide Polymorphism (SNPs) at Both Loci of rhg_1 and Rhg_4 in Soybean Resistant Germplasm

研究以 5 7份中美大豆抗胞囊线虫病种质资源为实验材料 ,利用基于检测微珠的单碱基延伸方法 ,对与大豆胞囊线虫病 (SCN)抗性基因rhg1和Rhg4紧密连锁的SNPs进行分析 ,目的是阐明我国大豆抗性种质在这两个位点的SNPs等位变异分布频率 ,为中国大豆种质抗SCN资源的利用奠定基础。分析结果表明 ,SNPs的抗性等位基因与中国大豆种质综合抗性的关系比不同生理小种的抗性关系更为密切。在rhg1和Rhg4位点 ,美国的 9份抗性种质中 ,有 7份抗性种质的SNPs均为纯合抗病基因型 ,而中国 4 8份抗性种质中有 32份 ,分别占鉴定总数的 77 8%和 6 6 7% ,推测大豆抗SCN种质中 ,以rhg1和Rhg4这两个基因协同作用表现出的抗性可能占多数 ,但还存在其他的抗性机制。

In this study, 57 resistant soybean collections from both China and US were used as materials. They were detect for SNPs at rhg 1 and Rhg 4 loci by using single based chain extension with a microsphere-based assay. The purpose was to illustrate the distribution of alleles at SNPs loci in Chinese accessions and provides the information for utilization of SCN resistant accessions in China. The comprehensive resistances of Chinese soybean SCN resistant accessions were more related to the SNPs genotypes compared to resistance for each SCN race. At both rhg 1 and Rhg 4 loci, 7 of 9 US resistant accessions appeared to be resistant genotypes, and 37 of 49 had resistant genotypes too, taking 77 8% and 66 7% of the total accessions, respectively. Based on this result, it was postulated that most of the Chinese resistant accessions in this study had resistance by interaction of both rhg 1 and Rhg 4, but the other resistant accessions may have different resistant mechanism.