几丁质酶基因导入西瓜植株及其抗病性鉴定研究

Transformation of Watermelon Plants with Chintinase Gene and Evaluation for Fusarium Wilt Resistance

利用西瓜有性生殖过程 ,将携带有外源几丁质酶基因的质粒DNA涂抹在授粉后的柱头上 ,使其沿花粉管通道进入到生殖细胞 ,得到转化处理种子。转化T1代植株经除草剂Basta筛选和PCR扩增获得转化植株。对T3 代株系进行田间自然发病和人工接种鉴定获得 3个抗枯萎病的株系。结果表明 :几丁质酶对镰刀菌引起的西瓜枯萎病有一定的抑制作用 ,利用花粉管通道法直接导入西瓜活体植株的技术是可行的。

Plasmid DNA with foreign chitinase gene was introduced into water melon lines by the approach of pollen-tube pathway. Transgenic plants were obtained by basta screening and PCR amplification.Three transgenic lines with improved resistance to Fusarium wilt were selected by natural occurrence and artificial inoculation. The results indicated that the chitinase had certain inhibition effect on Fusarium oxysporum, and the method of pollen tube pathway could be successfuly used in watermelon transformation.