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EB病毒蛋白对免疫球蛋白产生的调节作用 被引量:3

Regulation of immunoglobulin production with Epstein-Barr virus protein
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摘要 目的 :检验EB病毒 (Epstein BarrVirus,EBV)蛋白对培养的人B细胞产生免疫球蛋白 (Immunoglobulin ,Ig)的影响。方法 :用UV灭活和热处理的EBV刺激培养的人脐带血B细胞 ,流式细胞仪检测UV灭活EBV组细胞CD5、CD3、CD4和CD8的表达 ,ELISA法检测培养上清中IgG和IgM ,同时与EBV转化B细胞产生的IgG和IgM作对比。结果 :第 14天未检测到T细胞 ,CD5 + B细胞占 4 3% ;2 8天时CD5 + B细胞占 4 7%。UV灭活EBV组 :第 10、18、2 2和 2 6天IgGA值与对照组相比有显著差异 (P <0 0 5 ) ,第 10天以后各时间点IgMA值与对照组相比有显著差异 (P <0 0 5 )。EBV转化培养B细胞 4 0天 ,IgG和IgMA值与同期其它各组有显著差异 (P <0 0 5 )。结论 :EBV蛋白有诱导Ig产生的作用。 Objective:To investigate the effect of EBV protein on the production of Immunoglobulin(Ig)by cultured B cells.Methods:The purified human umbilical blood B cells were treated respectively with UV and Heat-inactivated EBV and cultured in complete IMDM. CD5, CD3, CD4 and CD8 cells were measured by flow cytometric analysis. IgG and IgM in the supernatant were detected by ELISA. At the mean time, IgG and IgM in the supernatant of cultured EBV-transformed B lymphocytes were detected by ELISA,and compared with that of UV-inactivated EBV group at the same period.Results:In UV-inactivated EBV group,CD3, CD4 and CD8 T cells couldn’t be detected,CD5 + cells occupied about 42% of all detected cells at the 14th day; and at the 28th day, 47%.The IgG A value of the 10th、18th、22th and 26th day in UV-inactivated EBV group have a significant difference compared with that in the control group ( P <0.05);The IgM A value of various time points after the 10th day in UV-inactivated EBV group have a significant difference( P <0.05). At the 40th day, IgG and IgM A value in EBV-transformed B cell group had significant difference compared with that in UV-inactivated EBV group( P <0.05).Conclusion:It is suggested that EBV protein is a key component to stimulate Ig production. These results make the base for further researching the effect of EBV gp350 on the production of natural autoantibodies.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2003年第8期524-526,529,共4页 Chinese Journal of Immunology
基金 国家自然科学基金资助项目 (3 0 170 862 )
关键词 EB病毒蛋白 免疫球蛋白 调节作用 B细胞 ELISA法 Immunoglobulin Epstein-Barr Virus CD5+B lymphocyte
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参考文献13

  • 1Miller G, Lipman M. Release of infectious Epstein-Barr virus by transformed marmoset leukocytes[J]. Proc Nat Acad Sci USA, 1973;70(1 ):190-194.
  • 2Gosselin J, Borgeat P. Epstein-Barr virus modulates 5-lipoxygenase product synthesis in human peripheral blood mononuclear cells[J]. Blood, 1997;89(6) :2122-2130.
  • 3Kimata H, Fujimoto M. Induction of IgA1 and IgA2 production in immature human fetal B cells and pre-B cells by vasoactive intestinal peptide[ J ].Blood, 1995;85(8) :2098-2104.
  • 4Mouthon L,Lacroxi-Desmazes S, Nobrega A et al. The self-reactive antibody repertoire of normal human serum IgM is acquired in early childhood and remains stable throughout life[J].Scand J Immunol, 1996;44(3):243-251.
  • 5Youinou P,Jamin C, Lydard P M.CD5 Expression in human B-cell populations[J]. Immunology Today, 1999;20(7) :312-316.
  • 6Paloczi K, Batai A. Immunophenotypic characterisation of cord blood B lymphocytes [ J ]. Bone Marrow Transplant, 1998; 22 ( Suppl 4 ) : S89-S91.
  • 7Daha M R, Bloem A C, Ballioax R E. Immunoglobulin production by human peripheral lymphocytes induced by anti-C3 receptor antibodies[J] .J Immunol, 1984; 132(3) : 1197-1201.
  • 8Hayakawa K, Asano M,Shinton S A et al. Positive selection of natural autoreactive B cells[J]. Science, 1999;285(5424) : 113-116.
  • 9Reid R R, Woodcock S.The role of compleement receptors CD21/CD35 in positive selection of B-1 cells[J]. Curr Top Microbiol Immunol,2000;252:57-65.
  • 10Lowell C A, Klickstein L B, Carter R H et al. Mapping of the epstein-barr virus and C3dg binding sites to a common domain on complement receptor type 2[J] .J Exp Med,1989;170(6) : 1931-1946.

同被引文献23

  • 1孙亚男,吴百灵.基于文献挖掘探讨原发性胆汁性胆管炎的中医用药规律[J].中医临床研究,2022,14(35):42-46. 被引量:1
  • 2王琳.CD21/CR2的研究进展[J].免疫学杂志,2003,19(S1):100-103. 被引量:8
  • 3Awameas S, Temynck T. Natural autoantibodies: The other side of the immune system[J]. Res Immunol, 1995,146(2):235-249.
  • 4Kasaian MT, Casali P. Autoimmunity-prone B-1 (CD5+ B)cells, natural antibodies and self recognition[J]. Autoimmunity, 1993,15(3) :315-329.
  • 5Paloczi K, Batai A. Immunophentypic characterization of cord blood B-lymphocytes[J]. Bone Marrow Transplant, 1998,22(suppl 4) :S89-S91.
  • 6Ishihara K, Hirano T. IL-6 in autoimmune disease and chronic inflammatory proliferative disease [J]. Cytokine Growth Factor Rev, 2002,13(4/5) :357-368.
  • 7Mario D, Towia AL, Jing WX, et al. Epstein-Barr virus and its glycoprotein-350 upregulate IL-6 in human B-lymphocytes via CD21, involving activation of NF-κB and different signaling pathways[J]. J Mol Biol, 2001,308(3) :501-514.
  • 8Bruce EG, Andrew L, Quirijin V. et al. Enhancde and sustained activation of human B cells by anti-immunoglobulin conjugated to the EBV glycoprotein gp350[J]. Eur J Immunol,2000,30(3) :969-973.
  • 9Reid RR, Woodcock S, Shimabukuro Vomhagen A, et al.Functional activity of natural antibody is altered in Cr2-deficient mice[J]. J Immunol,2002,169(10) :5 433-5 440.
  • 10Bandyopadhyay RS, Teutsh MR, Wortis HH. Activation of B-cells by sIgM cross-linking induces accumulation of CD5Mrna[J]. Curr Top Microbiol Immunol, 1999, 194 (2):219-228.

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