中性粒细胞小GTP结合蛋白的表达与冠心病发生关系的研究

Differential expression of Rac2 and RhoGDI in the neutrophil and its role in coronary heart disease

目的 :探讨中性粒细胞活性氧的产生及小GTP结合蛋白Rac2和RhoGDI的差异性表达在冠心病 (GHD)发生发展中的作用。方法 :采用化学发光法检测活性氧 ,适时定量RT PCR检测调控中性粒细胞活性氧产生的两种小GTP结合蛋白Rac2和RhoGDImRNA表达量的变化。结果 :冠心病组中性粒细胞产生活性氧显著增多 ,冠心病人血清中各种炎性因子有促进中性粒细胞产生活性氧的作用 ;Rac2mRNA在冠心病组表达量显著性高于正常对照组 ,而RhoGDImRNA的表达量在两组中无显著区别 ,Rac2mRNA和RhoGDImRNA表达量的比值与中性粒细胞产生活性氧的峰值呈正相关。结论 :中性粒细胞通过产生活性氧参与冠心病的发生发展 ,冠心病病人中性粒细胞Rac2和RhoGDImRNA表达量比例失调是导致活性氧产生增多的重要因素。

Objective:Evaluate the role of reactive oxygen species production and the differential expression of Rac2 and RhoGDI of neutrophils in pathogenesis of coronary heart disease.Methods:Reactive oxygen species production was measured by chemiluminescence method,the mRNA expression of Rac2 and RhoGDI was detected by quantitative RT-PCR.Results:The reactive oxygen species production by neutrophil is significantly increased in the coronary heart disease group,and the increase is related to the inflammatory cytokines in plasma.There is a significant increase of Rac2mRNA expression in coronary heart disease group than that of the control,while the expression of RhoGDImRNA in two groups are not significantly different.The ratio of Rac2mRNA to RhoGDImRNA is correlated to the production of reactive oxygen species of neutrophils.Conclusion:Netrophils are involved in the pathogenesis of coronary heart disease through their reactive oxygen species production,and the abnormal expression of Rac2 and RhoGDI is an important cause leading to the increased reactive oxygen species production.