液体悬浮培养促进蔓茎堇菜高效再生的研究

Study on the Rapid Plant Regeneration of Viola diffusa Ging.by Liquid Shake Culture

液体悬浮培养具有促进蔓茎堇菜芽分化的作用。以叶片为外植体在培养基MS +2 ,4 -D 1.5 +ZT 1.0中诱导出生长良好的愈伤组织。愈伤组织在液体培养基MS +6 -BA 1.0 +NAA 0 .2 5中振荡培养 12d后 (转速为 90r/min) ,转入固体培养基MS +6 -BA 2 .0 +NAA 0 .5中继续培养 14d ,芽分化率可达 97.5 %。转接至生根培养基MS +NAA 2 .0 +6 -BA 0 .2 5中培养 1个月后可移栽 ,成活率在 90 %以上。

The propagation of shoots of Viola diffusa Ging.can be promoted by liquid shake culture.Well-grown calli are induced from leaves in the media of MS+2,4-D 1.5+ZT 1.0.The calli are cultured in the solid media of MS+6-BA 2.0+NAA 0.5 for 14 days after liquid shake culture (90 r/min) in the liquid media of MS+6-BA 1.0+NAA 0.25 for 12 days,then the inducing rate of shoots is 97.5%.The plants can be transplanted after the shoots are cultured in the root media of MS+NAA 2.0+6-BA 0.25 for 1 month,and the living rate is over 90%.