耐热芽孢杆菌高温中性蛋白酶制备工艺研究

Study on the Pilot Preparation Technology of the XJT-9503 Thermostable Neutral Protease

为使耐热芽孢杆菌 (ThermophilicBacillus)XJT95 0 3高温中性蛋白酶产业化并在生产中应用 ,对XJT95 0 3高温中性蛋白酶的制备工艺进行了研究。结果表明 ,可采用加热真空薄膜浓缩 ,在 4 0℃ ,- 0 .0 94～ - 0 .0 96MPa真空度下 ,经过 4 5～ 5 5min浓缩 ,可使发酵处理液浓缩 2～ 3倍 ,其酶活力仅损失 10 %左右。确定了采用喷雾干燥法生产固体酶制剂的最优工艺条件 :进口温度170℃、出口温度 75℃、流量 30kg·h- 1 。在此条件下 ,酶得率为 6 8%。对固体酶与液体酶保存期稳定性的测定结果表明 ,固体酶的保存效果远比液体酶好 ,当保存 2 10d时 ,固体酶制剂的酶活损失仅为 2～ 8%。

In order to industrialize the XJT9503 thermostable neutral protease and make it applied in every area of industry.The preparation technology of the XJT9503 thermostable neutral protease had been studied.The experiment results indicated that the activity of the enzyme only lost about 10% when concentrated by using thermal spin concentrator,under the conditions of 40℃,-0.094～-0.096Mpa,after 45-55min concentrating,the concentration of the fermentation broth could be concentrated by 2-3 times of that of original.And the solid enzyme preparation technology-the spray-on process optimum conditions had been determined:the entrance temperature 170℃,the exit temperature 75℃,flow rate 30kg/h-1.At these conditions the enzyme productivity was 68%.Compared to the liquid enzyme,the solid enzyme was more stable than the liquid one.After storing 210 days,the activity of solid enzyme only lost about 2-8%.