膀胱癌AgNOR染色的研究

AgNORs study of bladder carcinoma

目的应用AgNOR技术判断膀胱癌的恶性程度及预后。方法对65例膀胱癌技术进行AgNOR染色,油镜下计数肿瘤细胞内AgNOR颗粒。结果14例膀胱癌平均计数AgNOR为380±0 63,正常组为1 65±0 18,两组有显著差异(P<0 01)。结论 AgNOR颗粒计数判断膀胱癌的恶性成度预后有一定价值。

Objective:To predict malignancy and prognosis in bladder carcinoma by the quantitative assessment of orgyrophilic nucleolar,organizer region AgNORs.Methods:The histologic sections of bs cases of bladder carcinoma were stained using AgNORs and the AgNORs granules in tumor ceus were counted with the help of oil-light microscope.Results:AgNORs of 14 cases of bladder carcinoma 380±063 was significantly higher(P<001).in comparision with normal group 165±018.AgNORs of 18 cases of bladder carcinoma at grade Ⅲ(399±030)was not significantly higher than AgNORs of 64 cases at grade Ⅰ-Ⅱ383±039(P<005).Conclusions:The deteetion of AgNORs is useful for predicting malignancy and prognosis in bladder carcinoma.