当归芍药散加味方对老年鼠松果体功能的影响

Effect of Supplemented Danggui Shaoyao San on Function of Pineal Body in Aged Rats

[目的]研究当归芍药散加味方(DSS)对老年鼠松果体功能的影响。[方法]40只老年鼠(18个月)分为对照组、全方组、君臣药组、佐药组,分组灌服DSS及拆方水煎液,3周后,取各组大鼠松果体进行培养,分离松果体培养上清液(PCS),然后加入到ConA诱导的脾细胞增殖培养中,用Ciemsa染色法和细胞计数测定脾细胞增殖数量和有丝分裂率、增殖率。[结果]老年对照组、全方组、君臣药组、佐药组有丝分裂率分别为(2.25±1.31)、(4.08±1.14)、(3.98±1.07)、(2.46±1.37),增殖率分别为(1.30±0.017)、(1.86±0.011)、(1.81±0.013)、(1.25±0.15)。与老年对照组比较,DSS全方组和君臣药组脾细胞有丝分裂率、增殖率明显增多(P<0.01)。[结论]结果表明服DSS可使老年鼠松果体分泌较多的促进淋巴细胞增殖的物质。

[Objective]To study the effect of supplemented Danggui Shaoyao San(DSS) on function of pineal body in aged rats. [Methods] Forty aged rats(18 months) were divided into Control group, DSS group, Junchen group and Zuo group, the rats were fed supplemented DSS or its separating prescription respectively for 21 days. Pineal bodies of rats in different groups were cultured. Pineal culture supernate (PCS) was separated. PCS was added to culture medium. Then, Con A was added to induce splenocyte proliferation. Mitoqenetic index and proliferation index were analyzed by Giemsa method. [Results] Mitogenetic of Control group, DSS group, Junchen group and Zuo group were 2. 25 ± 1. 31, 4. 08 ± 1. 14, 3. 98 ± 1. 07, 2. 46 ± 1. 37 respectively. Proliferation index in the four groups above were 1.30 +0.017, 1.86±0.011, 1.81±0.013, 1.25±0.15 respectively. Compared to the control group, mitogenetic index and proliferation index of DSS group and Junchen medicine group were showed significant increase (P <0.01). [Conclusion] The results show that DSS can help pineal body secrete more material to promote plenocytes proliferation.