摘要
AIM: To investigate the effect of cell adhesion molecule Pselectin monoclonal antibody (Mab) on metastasis and immune function of mice orthototopically implanted with human gastric cancer tissue.METHODS: SCID mice were implanted orthotopically with SGC-7901 human gastric carcinoma tissue. Starting from day 3 after operation, animals were given intravenously PBS or P-selectin Mab (100 μg/injection) (for both normal mice and tumor-implanted mice with tumors), twice weekly for 3weeks. Two animals in each group were sacrificed randomly at the 1st, 2nd, 4th week and 6th week. While T cell and B cell transformation indices were determined with the 3H TdR infiltration method, the NK cell activity was detected by the LDH release method.RESULTS: The metastatic rate in the P-selectin Mab treated group was lower than that in the PBS treated group (with tumors). The NK activity of normal mice increased over time.The immune functions (T, B cell function, NK activity) of the tumor group in the 6th week were significantly lower than those in the 4th week, but the change was attenuated by Pselectin Mab.CONCLUSION: P-selectin Mab could suppress the metastasis of gastric cancer with no adverse effect on host immune function.
AIM:To investigate the effect of cell adhesion molecule P- selectin monoclonal antibody (Mab) on metastasis and immune function of mice orthototopically implanted with human gastric cancer tissue. METHODS:SCID mice were implanted orthotopically with SGC-7901 human gastric carcinoma tissue.Starting from day 3 after operation,animals were given intravenously PBS or P-selectin Mab (100 μg/injection) (for both normal mice and tumor-implanted mice with tumors),twice weekly for 3 weeks.Two animals in each group were sacrificed randomly at the 1st,2nd,4th week and 6th week.While T cell and B cell transformation indices were determined with the ~3H TdR infiltration method,the NK cell activity was detected by the LDH release method. RESULTS:The metastatic rate in the P-selectin Mab treated group was lower than that in the PBS treated group (with tumors).The NK activity of normal mice increased over time. The immune functions (T,B cell function,NK activity) of the tumor group in the 6th week were significantly lower than those in the 4th week,but the change was attenuated by P- selectin Mab. CONCLUSION:P-selectin Mab could suppress the metastasis of gastric cancer with no adverse effect on host immune function.
基金
the Youth Science Foundation of Shanghai Public Administration No.131984Y15
