摘要
AIM: To investigate the expression of local renin and angiotensinogen mRNA in cirrhotic portal hypertensive patients.METHODS: The expression of local renin and angiotensinogen mRNA in the liver, splenic artery and vein of PH patients was detected by RT-PCR analysis.RESULTS: Expression of local renin mRNA in the liver of control group was (0.19±0.11), significantly lower than that in splenic artery(0.45±0.17)or splenic vein(0.39±0.12)respectively, (P＜0.05). Expression of local angiotensinogen mRNA in the liver was (0.64±0.21), significantly higher than that in splenic artery(0.41±0.15) or in splenic vein (0.35±0.18)respectively, (P＜0.05). Expression of local renin mRNA in the liver, splenic artery and vein of PH group was (0.78±0.28),(0.86±0.35) and (0.81±0.22) respectively, significantly higher than that in the control group, (P＜0.05). Expression of local angiotensinogen mRNA in the liver, splenic artery and vein of PH group was (0.96±0.25), (0.83±0.18) and (0.79±0.23)respectively, significantly higher than that in the control group,(P＜0.05). There was no significant difference between the liver, splenic artery and vein in the expression of local renin or local angiotensinogen mRNA in PH group, (P＜0.05).CONCLUSION: In normal subjects the expression of local renin and angiotensinogen mRNA was organ specific, but with increase of the expression of LRAS, the organ-specificity became lost in cirrhotic patients. LRAS may contribute to increased resistance of portal vein with liver and formation of splanchnic vasculopathy.
AIM:To investigate the expression of local renin and angiotensinogen mRNA in cirrhotic portal hypertensive patients. METHODS:The expression of local renin and angiotensinogen mRNA in the liver,splenic artery and vein of PH patients was detected by RT-PCR analysis. RESULTS:Expression of local renin mRNA in the liver of control group was (0.19±0.11),significantly lower than that in splenic artery(0.45±0.17)or splenic vein(0.39±0.12) respectively,(P<0.05).Expression of local angiotensinogen mRNA in the liver was (0.64±0.21),significantly higher than that in splenic artery(0.41±0.15) or in splenic vein (0.35±0.18) respectively,(P<0.05).Expression of local renin mRNA in the liver,splenic artery and vein of PH group was (0.78±0.28), (0.86±0.35) and (0.81±0.22) respectively,significantly higher than that in the control group,(P<0.05).Expression of local angiotensinogen mRNA in the liver,splenic artery and vein of PH group was (0.96±0.25),(0.83±0.18) and (0.79±0.23) respectively,significantly higher than that in the control group, (P<0.05).There was no significant difference between the liver,splenic artery and vein in the expression of local renin or local angiotensinogen mRNA in PH group,(P<0.05). CONCLUSION:In normal subjects the expression of local renin and angiotensinogen mRNA was organ specific,but with increase of the expression of LRAS,the organ-specificity became lost in cirrhotic patients.LRAS may contribute to increased resistance of portal vein with liver and formation of splanchnic vasculopathy.
基金
the National Natural Science Foundation of China,No 30170920
