摘要
AIM: To study the effects of carbon dioxide on the metastatic capability of cancer cells, and to compare them with that of nitrogen.METHODS: The colon cancer cell CCL-228 was treated with 100 % carbon dioxide or nitrogen at different time points and then cultured under normal condition. Twelve hours after the treatment, the survival rates of suspension cells and the expressions of e-cadherin and VEGF were examined.RESULTS: After 60 min of carbon dioxide and longer time of nitrogen treatment, the suspended cells increased and the expression of e-cadherin decreased while the expression of VEGF was enhanced significantly. And the effects of nitrogen were similar to, but weaker than, those of carbon dioxide.CONCLUSION: Carbon dioxide may improve the metastatic capability of cancer cells and its effects are significantly stronger than that of nitrogen. A sequential use of carbon dioxide and nitrogen in pneumoperitoneum may take the advantage of both gases.
AIM:To study the effects of carbon dioxide on the metastatic capability of cancer cells,and to compare them with that of nitrogen. METHODS:The colon cancer cell CCL-228 was treated with 100% carbon dioxide or nitrogen at different time points and then cultured under normal condition.Twelve hours after the treatment,the survival rates of suspension cells and the expressions of e-cadherin and VEGF were examined. RESULTS:After 60 min of carbon dioxide and longer time of nitrogen treatment,the suspended cells increased and the expression of e-cadherin decreased while the expression of VEGF was enhanced significantly.And the effects of nitrogen were similar to,but weaker than,those of carbon dioxide. CONCLUSION:Carbon dioxide may improve the metastatic capability of cancer cells and its effects are significantly stronger than that of nitrogen.A sequential use of carbon dioxide and nitrogen in pneumoperitoneum may take the advantaqe of both qases.
基金
the Natural Science Foundation of Hubei Province,No.2000J062
