摘要
Purpose: To observe the effect of acupuncture on the brain-taxis of tetramethylpyrazine (TMP) and to explore into the underlying mechanisms of combined action of acupuncture and medicine in the treatment of acute cerebral ischemia. Methods: 37 male Wistar rats were randomly divided into normal control group (n=10), sham-operation group (n=10), acute cerebral ischemia (ACI) + drug group (model group, n=8)and ACI+drug+acupuncture group (acupuncture group, n=9). Rat ACI model was established by using photochemical method. "Neiguan"(PC 6) and "Shuigou"(GV 26) were punctured and stimulated with both hand manipulation and electroacupuncture, 30 min and 16hrs after ACI. TMP was given to the rats of the later 2 groups using gastric perfusion method. High pressure chromatography (HPLC) was used to detect the target absorption level of TMP in the brain. Results: The content of TMP in the brain in acupuncture group was significantly higher than that in model group (P<0.01), suggesting that acupuncture can strengthen the brain-taxis of TMP in ACI rats, and combined administration of acupuncture and Chinese drug maybe work better for treatment of acute cerebral infarction. Conclusion: Acupuncture can strengthen the chemo-taxis of TMP to the brain in ACI rats.
Purpose: To observe the effect of acupuncture on the brain-taxis of tetramethylpyrazine (TMP) and to explore into the underlying mechanisms of combined action of acupuncture and medicine in the treatment of acute cerebral ischemia. Methods: 37 male Wistar rats were randomly divided into normal control group (n=10), sham-operation group (n=10), acute cerebral ischemia (ACI) + drug group (model group, n=8)and ACI+drug+acupuncture group (acupuncture group, n=9). Rat ACI model was established by using photochemical method. 'Neiguan'(PC 6) and 'Shuigou'(GV 26) were punctured and stimulated with both hand manipulation and electroacupuncture, 30 min and 16hrs after ACI. TMP was given to the rats of the later 2 groups using gastric perfusion method. High pressure chromatography (HPLC) was used to detect the target absorption level of TMP in the brain. Results: The content of TMP in the brain in acupuncture group was significantly higher than that in model group (P<0.01), suggesting that acupuncture can strengthen the brain-taxis of TMP in ACI rats, and combined administration of acupuncture and Chinese drug maybe work better for treatment of acute cerebral infarction. Conclusion: Acupuncture can strengthen the chemo-taxis of TMP to the brain in ACI rats.
基金
ThissubjectwassupportedbytheScienceCommitteeofLiaoningProvince (No .0 0 2 0 2 9)
