碱性成纤维细胞生长因子对海马伞切断大鼠海马神经的影响(英文)

Effect of basic fibroblast growth factor on hippocampus neurons in rat model with unilateral fimbria fornix transection

目的了解碱性成纤维细胞生长因子(bFGF)对海马伞切断造成的阿尔茨海默病(AD)模型大鼠海马齿状回神经发生的影响,探讨bFGF治疗AD的前景。方法36只SD大鼠随机分成正常对照组(n=12)、AD对照组(n=12)和AD处理组(n=12)。AD处理组及AD对照组大鼠左侧海马伞切断制造AD模型;正常对照组注射生理盐水。AD处理组造模后每天侧脑室注射bFGF共7d;AD对照组及正常对照组同时间注射生理盐水。BrdU标记增殖细胞。TUNEL方法标记DNA片段,原位检测凋亡细胞。计数海马齿状回BrdU阳性细胞与凋亡细胞数。结果AD处理组大鼠与AD对照组大鼠相比,海马齿状回BrdU阳性细胞增加犤两组在颗粒细胞层阳性细胞分别为(163±37),(53±5)个/切片平面;海马门(28.5±5.5),(12.3±2.8)个/切片平面;分子层(10.7±2.2),(6.0±1.4)个/切片平面犦,差异有非常显著性意义(F=103.4,83.4,33.4,P<0.01),而凋亡细胞差异无显著性意义(P>0.05)。AD对照组大鼠与正常对照组大鼠相比,海马齿状回BrdU阳性细胞数及凋亡细胞差异均无显著性意义(P>0.05)。结论bFGF可刺激AD模型大鼠海马神经发生。

Aim To study the effect of basic fibroblast growth factor(bFGF) on neurogenesis in dentate gyrus of rat model of AD and to explore the application prospect of bFGF in treatment of AD with unilateral fimbria fornix transection.Methods 36 SD rats were randomly divided into normal control,AD control and AD treatment groups with 12 rats in each group.We established rat model of AD via left fimbria fornix transection.Normal saline was injected in normal control group.Rats in AD treatment group bFGF infusion into the cerebroventricular space every day after being lesioned for 7 days.Normal saline was injected in AD control group and normal control group.BrdU was used to label proliferated cells.DNA segments were labeled by TUNEL method and apoptic cells were detected in situ.Numbers of positive and apoptic cells at dentate gyrus were counted.Results Compared with AD control group,number of BrdU positive cells increased in AD treatment groupEffect of basic fibroblast growth factor on hippocampus neurons in rat model with unilateral fimbria fornix transection$$$$ Si Lang Zhou, Jun Pao Chen, Dong Lin Cao, Xiao Wen Tu, Dai Jun YuanSi Lang Zhou, Jun Pao Chen, Dong Lin Cao, Xiao Wen Tu, Dai Jun Yuan,Department of Neurology,Zhujiang Hosipital,the First Military Medical Univerisity,Guangzhou 510282,ChinaABSTRACT:Aim To study the effect of basic fibroblast growth factor(bFGF) on neurogenesis in dentate gyrus of rat model of AD and to explore the application prospect of bFGF in treatment of AD with unilateral fimbria fornix transection.Methods 36 SD rats were randomly divided into normal control,AD control and AD treatment groups with 12 rats in each group.We established rat model of AD via left fimbria fornix transection.Normal saline was injected in normal control group.Rats in AD treatment group bFGF infusion into the cerebroventricular space every day after being lesioned for 7 days.Normal saline was injected in AD control group and normal control group.BrdU was used to label proliferated cells.DNA segments were labeled by TUNEL method and apoptic cells were detected in situ.Numbers of positive and apoptic cells at dentate gyrus were counted.Results Compared with AD control group,number of BrdU positive cells increased in AD treatment group[granular cell layer, ( 163± 37) vs ( 53± 5) /section plane;hippocampus hilum, ( 28.5± 5.5) vs ( 12.3± 2.8) /section plane;molecular layer, ( 10.7± 2.2) vs ( 6.0± 1.4) /section plane,significant differences existed( F=103.4, 83.4, 33.4, P< 0.01) while no significant difference was found in number of apoptic cells( P >0.05) .There were no significant differences in number of BrdU positive cells and apoptic cells at dentate gyrus compared between AD control group and normal control group.Conclusion bFGF can promote neurogenesis in dentate gyrus of rat model of AD.