摘要
目的研究以麻黄、山栀子、黄连、厚朴、白术、桂枝、三七为主要成分的中药复方护脑素改善脑部微循环及脑保护的作用机制。方法将Wistar大鼠随机分成3组,模型组(结扎两侧颈总动脉)、护脑素组(颈总动脉结扎前半小时腹腔灌注护脑素混悬液)和假手术组,每组10只。用组织血流量仪测定各组手术前后局部脑血流量(r-CBF),用硝酸还原法测定血浆一氧化氮(NO),用放射免疫分析法检测血浆内皮素1(ET-1)。手术后2h将大鼠处死,取出脑组织,测定脑匀浆中NO与ET-1含量。结果①rCBF值:模型组为(202±38)mL/(min·kg),显著低于假手术组(635±81)mL/(min·kg)(t=15.34,P<0.001)和护脑素组(267±47)mL/(min·kg)(t=3.33,P<0.05)。②护脑素组血浆NO(2.6±0.5)mol/L高于模型组(2.1±0.8)mol/L(t=4.23,P<0.05)。模型组血浆ET-1(540.0±101.0)ng/L高于假手术组(349.0±26.0)ng/L,(t=6.14,P<0.05)和护脑素组(427.0±83.0)ng/L,(t=2.73,P<0.05)。③模型组组织匀浆中NO(406.0±163.5)mol/L和ET-1(11.4±2.0)ng/L分别高于假手术组的(177.4±63.3)mol/L,(8.1±1.5)ng/L,(t=4.13,4.04,P<0.005)和护脑素组的(207.2±113.4)mol/L,(8.3±1.7)ng/L(t=3.16,3.64,P<0.005)。结论护脑素在急性脑缺血模型中可通过NO、ET-1的参与,改善脑部微循环。
Aim To study the mechanism of Chinese medicine compound Hu Nao Su, with ephedra, pittosporm root, coptis root, magnolia bark, bighead atractylodes rhizome, cinnamom twig, and notoginseng as its essential components, in promoting cerebral microcirculation and protecting brain cells in acute cerebral ischemia so as to evaluate the therapeutic effects of Hu Nao Su in acute ischemia.Methods Thirty Wistar rats were randomly divided into three groups of 10 rats: model group (the bilateral common carotid arteries were ligated so as to construct a model of acute cerebral ischemia), pseudo operation group, and Hu Nao Su group (suspension of Hu Nao Su was infused intraperitoneally 30 min before the ligation of bilateral common carotid arteries).The regional cerebral blood flow (r CBF) before and after operation was detected by blood flow meter. The plasma content of nitric oxide (NO) was examined by nitrate reduction method; the plasma endothelin 1(ET 1) was examined by radioimmunoassay.Two hours after the operation the rats were killed and their brains were taken. The contents of NO and ET 1 in the brain homogenate were examined.Results ① The content of r CBF was (202± 38) mL/(min· kg) in the model group, obviously lower than that of the pseudo operation group[(635± 81) mL/(min· kg)] and Hu Nao Su group (267± 47)mL/(min· kg)] (t=15.34,P< 0.001; t=3.33,P< 0.05 respectively). ② The plasma NO content of the Hu Nao Su group was (2.6± 0.5) mol/L, significantly higher than that of the model group(2.1± 0.8) mol/L,t=4.23,P< 0.05]. The plasma ETl in the model group was (t=15.34,P< 0.001; t=3.33,P< 0.05 respectively). ② The plasma NO content of the Hu Nao Su group was (2.6± 0.5) mol/L, significantly higher than that of the model group(2.1± 0.8) mol/L,t=4.23,P< 0.05]. The plasma ETl in the model group was . The plasma ETl in the model group was [(540.0± 101.0) ng/L], significantly higher than that of the pseudo operation group[(349.0± 26.0) ng/L,t=6.14,P< 0.05], and Hu Nao Su group[(427.0± 83.0) ng/L, t=2.73,P< 0.05].③ The NO and ET 1 in the brain tissues of model group were (406.0± 163.5) mmol/mL and (11.4± 2.0)ng/L respectively, significantly higher than those of the pseudo operation group [(177.4± 63.3) mol/L and (8.1± 1.6) ng/L,t=4.13, 4.04,P< 0.005], and Hu Nao Su group[(207.2± 113.4) mmol/ mL and (8.3± 1.7) ng/ L,t=3.16, 3.64,P< 0.005].Conclusions Hu Nao Su promotes the cerebral microcirculation and protects the brain tissue through the involvement of NO and ET 1.
出处
《中国临床康复》
CSCD
2003年第19期2656-2657,共2页
Chinese Journal of Clinical Rehabilitation