白鲜皮药材指纹图谱研究

The HPLC/UV Fingerprint Analysis of Cortex Dictamni

目的 :采运用反相高效液相色谱法 ,研究并建立白鲜皮药材的指纹图谱。方法 :用Kromasil 1 0 0C1 8色谱柱 ,乙腈 水梯度洗脱 ;流速 1 0ml/min ;检测波长 2 2 8nm。结果 :运用梯度洗脱得到的色谱图各色谱峰分离较好 ,达到指纹图谱的要求。结论

AIM:To study and establish the fingerprint of raw Cortex Dictamni by reversed phased high performance liquid chromatography. METHOD:The chromatographic conditions were as follows: a Kromasil 100 C 18 column was used; the mobile phase was composed of acetontrile and water with gradient elution;the flow rate was 1 0 ml/min and the UV absorbance detection was set at 228 nm.RESULT:Under the selected chromatographic conditions, good HPLC fingerprints of Cortex Dictamni were obtained .CONCLUSION:Quality of raw Cortex Dictamni can be controlled effectively by HPLC UV fingerprint.