自制含不同磷、钙量的大鼠实验饲料的研究

Feeds with different phosphorus and calcium contents for laboratory rats

目的 探索自制适合磷代谢、继发性甲状旁腺功能亢进研究的含不同磷、钙剂量的大鼠饲料。方法 适当调整AIN - 93G标准配方 ,自制含不同磷、钙剂量饲料并检测鉴定含量 ,然后分别用高、低磷饲料喂饲慢性肾衰竭 (CRF)和假手术对照大鼠 2周 ;分别测定血离子钙 (iCa)、磷 (P)、甲状旁腺激素 (iPTH)、活性维生素D3 。结果 高磷饲料含磷 1.2 %、钙 1.6 % ,低磷饲料含磷 0 .2 %、钙 0 .5 % ;喂饲大鼠 2周后 ,高磷CRF组较低磷CRF组血磷 (3.93± 0 .2 7)vs (2 .4 2± 0 .84 )mmol/L ,P <0 .0 5 )及iPTH(75 .5 0± 2 0 .19)vs(30 .5 5± 2 .33) ,P <0 .0 5 )明显上升 ,两组血离子钙 (1.15± 0 .0 8)vs (1.2 0± 0 .0 8)mmol/L ,P >0 .0 5 )及活性维生素D3 (6 9.77± 10 .85 )vs (87.5 1± 8.6 3)pg/ml,P >0 .0 5 )无明显差异。结论 本配方能提供慢性肾衰竭磷代谢实验研究所需的高、低大鼠磷饲料 ,具有应用和推广价值。

Objective To study the laboratory rat's feeds with different phosphorus(P) and calcium(Ca) contents for research on the metabolism of P.Methods AIN-93G formulat was modified partly to manufacture the feeds with different P and Ca contents. The high-P and low-P feeds were fed to the CRF rats or sham operation control rats respectively for two weeks.The levels of serum ionized calcium,phosphorus, iPTH and 1,25-(OH) 2D 3 were observed.Results The high-P diet contains 1.2% P and 1.6% Ca,and the low one contains 0.2% P and 0.5% Ca. After two weeks, serum P and iPTH levels in CRF rats fed with high-P feeds were significantly higher than those of CRF rats fed with low-P feeds (P 3.93±0.27 vs 2.42±0.84 mmol/L, P<0.05; PTH 75.50 ± 20.19 vs 30.55 ± 2.33pg/ml, P<0.05, respectively), but there were no significant difference in serum ionized calcium and 1,25-(OH) 2D 3. Conclusion The formula of feeds for the laboratory rats in this experiment can fit for the requirement of research on the phosphorus metabolism.