抗心肌肌凝蛋白抗体亲大鼠梗塞心肌特性的研究

Study on characteristics of anticardiac myosin monoclonal antibody uptake in infarcted myocardium in rats

目的 探讨抗心肌肌凝蛋白单克隆抗体 (antimyosinantibody ,AMA)亲梗塞心肌的特点。方法 心肌梗死大鼠静脉注射放射性锝 99m标记的抗心肌肌凝蛋白单克隆抗体 (99mTc AMA) ,观察注射时间 ,梗死时间和梗塞区对梗塞心肌摄取AMA的影响。结果 注射后 2h梗塞心肌开始摄取AMA ,以后摄取逐渐增加 ,2 4h达到高峰。心肌梗死后 2 0d ,梗塞心肌持续摄取AMA ,期间心肌梗死后 1～ 5d摄取最强 ,梗塞中央区以及梗塞区内层摄取强于其他梗塞区域。结论 急性心肌梗死大鼠梗塞心肌特异性地摄取AMA ,注射AMA后摄取迅速、持久 ,受心肌梗死时间影响较小 ,梗塞中央区内层心肌摄取最强 ,AMA具有亲梗塞心肌的特性。

Objective To study the characteristics of anticardic myosin monoclonal antibody(AMA) uptake in infarcted myocardium in rats.Methods The ratio between infarcted and normal myocardium was calculated after intravenous injection of certain dose of 99mTc-AMA in the rats with experimental myocardial infarction and the influence of injection time,infarction time and zone of infarcted myocardium on the radioa-ctivity uptake in infarcted myocardium was observed.Results The infarcted myocardium took up AMA rapidly in the first 2 h.The uptake increased towards steady at 4～8 h and reached the peak at 24 h after injection.Compared with the ratio of N-IgG in the infarcted to normal myocardium,the ratio of AMA was significantly greater at the same time after injection(P<0.01).There was large uptake of AMA in the infarcted myocardium on days 1-20 during acute myocardium infarction(AMI).The uptake of AMA in the endocardial layer of the infarct center was greater than that in other region of infarcted myocardium.Conclusion The uptake of AMA may be specific,early,and long lasting in the infarcted myocardium and be little affected by infarct time.This may become a new approach to myocardial infarct imaging.